Optical Activation of TrkB (E281A) in Excitatory and Inhibitory Neurons of the Mouse Visual Cortex

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dc.contributor.authorLilja, Antoniako
dc.contributor.authorDidio, Giulianoko
dc.contributor.authorHong, Jongryulko
dc.contributor.authorHeo, Won Doko
dc.contributor.authorCastren, Eeroko
dc.contributor.authorUmemori, Juzohko
dc.date.accessioned2022-10-04T08:00:34Z-
dc.date.available2022-10-04T08:00:34Z-
dc.date.created2022-10-04-
dc.date.created2022-10-04-
dc.date.created2022-10-04-
dc.date.issued2022-09-
dc.identifier.citationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.23, no.18-
dc.identifier.issn1661-6596-
dc.identifier.urihttp://hdl.handle.net/10203/298802-
dc.description.abstractThe activation of tropomyosin receptor kinase B (TrkB), the receptor of brain-derived neurotrophic factor (BDNF), plays a key role in induced juvenile-like plasticity (iPlasticity), which allows restructuring of neural networks in adulthood. Optically activatable TrkB (optoTrkB) can temporarily and spatially evoke iPlasticity, and recently, optoTrkB (E281A) was developed as a variant that is highly sensitive to light stimulation while having lower basal activity compared to the original optoTrkB. In this study, we validate optoTrkB (E281A) activated in alpha calcium/calmodulin-dependent protein kinase type II positive (CKII+) pyramidal neurons or parvalbumin-positive (PV+) interneurons in the mouse visual cortex by immunohistochemistry. OptoTrkB (E281A) was activated in PV+ interneurons and CKII+ pyramidal neurons with blue light (488 nm) through the intact skull and fur, and through a transparent skull, respectively. LED light stimulation significantly increased the intensity of phosphorylated ERK and CREB even through intact skull and fur. These findings indicate that the highly sensitive optoTrkB (E281A) can be used in iPlasticity studies of both inhibitory and excitatory neurons, with flexible stimulation protocols in behavioural studies.-
dc.languageEnglish-
dc.publisherMDPI-
dc.titleOptical Activation of TrkB (E281A) in Excitatory and Inhibitory Neurons of the Mouse Visual Cortex-
dc.typeArticle-
dc.identifier.wosid000858322000001-
dc.identifier.scopusid2-s2.0-85138892602-
dc.type.rimsART-
dc.citation.volume23-
dc.citation.issue18-
dc.citation.publicationnameINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES-
dc.identifier.doi10.3390/ijms231810249-
dc.contributor.localauthorHeo, Won Do-
dc.contributor.nonIdAuthorLilja, Antonia-
dc.contributor.nonIdAuthorDidio, Giuliano-
dc.contributor.nonIdAuthorHong, Jongryul-
dc.contributor.nonIdAuthorCastren, Eero-
dc.contributor.nonIdAuthorUmemori, Juzoh-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorneural plasticity-
dc.subject.keywordAuthortropomyosin kinase receptor B-
dc.subject.keywordAuthorparvalbumin-positive interneurons-
dc.subject.keywordAuthorcalcium-
dc.subject.keywordAuthorcalmodulin positive pyramidal neurons-
dc.subject.keywordAuthorV1-
dc.subject.keywordAuthorvisual cortex-
dc.subject.keywordAuthormultiple regression with interaction and simple slopes analysis-
dc.subject.keywordPlusPLASTICITY-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusVECTOR-
dc.subject.keywordPlusKINASE-
dc.subject.keywordPlusBRAIN-
dc.subject.keywordPlusAAV-
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