Multiscale Functional Metal Architectures by Antibody-Guided Metallization of Specific Protein Assemblies in Ex Vivo Multicellular Organisms

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Biological systems consist of hierarchical protein structures, each of which has unique 3D geometries optimized for specific functions. In the past decades, the growth of inorganic materials on specific proteins has attracted considerable attention. However, the use of specific proteins as templates has only been demonstrated in relatively simple organisms, such as viruses, limiting the range of structures that can be used as scaffolds. This study proposes a method for synthesizing metallic structures that resemble the 3D assemblies of specific proteins in mammalian cells and animal tissues. Using 1.4 nm nanogold-conjugated antibodies, specific proteins within cells and ex vivo tissues are labeled, and then the nanogold acts as nucleation sites for growth of metal particles. As proof of concept, various metal particles are grown using microtubules in cells as templates. The metal-containing cells are applied as catalysts and show catalytic stability in liquid-phase reactions due to the rigid support provided by the microtubules. Finally, this method is used to produce metal structures that replicate the specific protein assemblies of neurons in the mouse brain or the extracellular matrices in the mouse kidney and heart. This new biotemplating approach can facilitate the conversion of specific protein structures into metallic forms in ex vivo multicellular organisms.
Publisher
WILEY-V C H VERLAG GMBH
Issue Date
2022-09
Language
English
Article Type
Article
Citation

ADVANCED MATERIALS, v.34, no.35

ISSN
0935-9648
DOI
10.1002/adma.202200408
URI
http://hdl.handle.net/10203/298487
Appears in Collection
RIMS Journal PapersMS-Journal Papers(저널논문)
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