DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeon, Jehwi | ko |
dc.contributor.author | Kim, Sang-Hoon | ko |
dc.contributor.author | Kong, Eunji | ko |
dc.contributor.author | Kim, Soo Jin | ko |
dc.contributor.author | Yang, Jee Myung | ko |
dc.contributor.author | Lee, Joo Yong | ko |
dc.contributor.author | Lee, Junyeop | ko |
dc.contributor.author | Kim, You-Me | ko |
dc.contributor.author | Kim, Pilhan | ko |
dc.date.accessioned | 2022-08-09T03:01:45Z | - |
dc.date.available | 2022-08-09T03:01:45Z | - |
dc.date.created | 2022-08-09 | - |
dc.date.issued | 2022-07 | - |
dc.identifier.citation | FRONTIERS IN MEDICINE, v.9 | - |
dc.identifier.issn | 2296-858X | - |
dc.identifier.uri | http://hdl.handle.net/10203/297892 | - |
dc.description.abstract | Animal models of retinal artery occlusion (RAO) have been widely used in many studies. However, most of these studies prefer using a central retinal artery occlusion (CRAO) which is a typical global ischemia model of the retina, due to the technical limitation of producing single vessel targeted modeling with real-time imaging. A focal ischemia model, such as branch retinal artery occlusion (BRAO), is also needed for explaining interactions, including the immunological reaction between the ischemic retina and adjacent healthy retina. Accordingly, a relevant model for clinical RAO patients has been demanded to understand the pathophysiology of the RAO disease. Herein, we establish a convenient BRAO mouse model to research the focal reaction of the retina. As a photo-thrombotic agent, Rose bengal was intravenously injected into 7 week-old transgenic mice (CX3CR1-GFP) for making embolism occlusion, which causes pathology similarly to clinical cases. In an optimized condition, a 561 nm laser (13.1 mw) was projected to a targeted vessel to induce photo-thrombosis for 27 s by custom-built retinal confocal microscopy. Compared to previous BRAO models, the procedures of thrombosis generation were naturally and minimal invasively generated with real-time retinal imaging. In addition, by utilizing the self-remission characteristics of Rose bengal thrombus, a reflow of the BRAO with immunological reactions of the CX3CR1-GFP(+) inflammatory cells such as the retinal microglia and monocytes was monitored and analyzed. In this models, reperfusion began on day 3 after modeling. Simultaneously, the activation of CX3CR1-GFP(+) inflammatory cells, including the increase of activation marker and morphologic change, was confirmed by immunohistochemical (IHC) staining and quantitative real-time PCR. CD86 and Nox2 were prominently expressed on day 3 after the modeling. At day 7, blood flow was almost restored in the large vessels. CX3CR1-GFP(+) populations in both superficial and deep layers of the retina also increased around even in the BRAO peri-ischemic area. In summary, this study successfully establishes a reproducible BRAO modeling method with convenient capabilities of easily controllable time points and selection of a specific single vessel. It can be a useful tool to analyze the behavior of inflammatory cell after spontaneous arterial recanalization in BRAO and further investigate the pathophysiology of BRAO. | - |
dc.language | English | - |
dc.publisher | FRONTIERS MEDIA SA | - |
dc.title | Establishment of the reproducible branch retinal artery occlusion mouse model and intravital longitudinal imaging of the retinal CX3CR1-GFP(+) cells after spontaneous arterial recanalization | - |
dc.type | Article | - |
dc.identifier.wosid | 000834620000001 | - |
dc.identifier.scopusid | 2-s2.0-85135173469 | - |
dc.type.rims | ART | - |
dc.citation.volume | 9 | - |
dc.citation.publicationname | FRONTIERS IN MEDICINE | - |
dc.identifier.doi | 10.3389/fmed.2022.897800 | - |
dc.contributor.localauthor | Kim, You-Me | - |
dc.contributor.localauthor | Kim, Pilhan | - |
dc.contributor.nonIdAuthor | Kim, Soo Jin | - |
dc.contributor.nonIdAuthor | Yang, Jee Myung | - |
dc.contributor.nonIdAuthor | Lee, Joo Yong | - |
dc.contributor.nonIdAuthor | Lee, Junyeop | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | branch retinal artery occlusion (BRAO) | - |
dc.subject.keywordAuthor | mouse modeling | - |
dc.subject.keywordAuthor | ischemic-reperfusion injury | - |
dc.subject.keywordAuthor | retinal microglia | - |
dc.subject.keywordAuthor | intravital imaging | - |
dc.subject.keywordPlus | ISCHEMIA-REPERFUSION INJURY | - |
dc.subject.keywordPlus | RAT RETINA | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | THROMBOLYSIS | - |
dc.subject.keywordPlus | DEGENERATION | - |
dc.subject.keywordPlus | MICROGLIA | - |
dc.subject.keywordPlus | STROKE | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | VEIN | - |
dc.subject.keywordPlus | TIME | - |
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