DC Field | Value | Language |
---|---|---|
dc.contributor.author | Fozouni, Parinaz | ko |
dc.contributor.author | Son, Sungmin | ko |
dc.contributor.author | Derby, Maria Diaz de Leon | ko |
dc.contributor.author | Knott, Gavin J. | ko |
dc.contributor.author | Gray, Carley N. | ko |
dc.contributor.author | D'Ambrosio, Michael, V | ko |
dc.contributor.author | Zhao, Chunyu | ko |
dc.contributor.author | Switz, Neil A. | ko |
dc.contributor.author | Kumar, G. Renuka | ko |
dc.contributor.author | Stephens, Stephanie, I | ko |
dc.contributor.author | Boehm, Daniela | ko |
dc.contributor.author | Tsou, Chia-Lin | ko |
dc.contributor.author | Shu, Jeffrey | ko |
dc.contributor.author | Bhuiya, Abdul | ko |
dc.contributor.author | Armstrong, Maxim | ko |
dc.contributor.author | Harris, Andrew R. | ko |
dc.contributor.author | Chen, Pei-Yi | ko |
dc.contributor.author | Osterloh, Jeannette M. | ko |
dc.contributor.author | Meyer-Franke, Anke | ko |
dc.contributor.author | Joehnk, Bastian | ko |
dc.contributor.author | Walcott, Keith | ko |
dc.contributor.author | Sil, Anita | ko |
dc.contributor.author | Langelier, Charles | ko |
dc.contributor.author | Pollard, Katherine S. | ko |
dc.contributor.author | Crawford, Emily D. | ko |
dc.contributor.author | Puschnik, Andreas S. | ko |
dc.contributor.author | Phelps, Maira | ko |
dc.contributor.author | Kistler, Amy | ko |
dc.contributor.author | DeRisi, Joseph L. | ko |
dc.contributor.author | Doudna, Jennifer A. | ko |
dc.contributor.author | Fletcher, Daniel A. | ko |
dc.contributor.author | Ott, Melanie | ko |
dc.date.accessioned | 2022-08-09T02:00:15Z | - |
dc.date.available | 2022-08-09T02:00:15Z | - |
dc.date.created | 2022-08-09 | - |
dc.date.created | 2022-08-09 | - |
dc.date.issued | 2021-01 | - |
dc.identifier.citation | CELL, v.184, no.2, pp.323 - 333 | - |
dc.identifier.issn | 0092-8674 | - |
dc.identifier.uri | http://hdl.handle.net/10203/297866 | - |
dc.description.abstract | The December 2019 outbreak of a novel respiratory virus, SARS-CoV-2, has become an ongoing global pandemic due in part to the challenge of identifying symptomatic, asymptomatic, and pre-symptomatic car riers of the virus. CRISPR diagnostics can augment gold-standard PCR-based testing if they can be made rapid, portable, and accurate. Here, we report the development of an amplification-free CRISPR-Cas13a assay for direct detection of SARS-CoV-2 from nasal swab RNA that can be read with a mobile phone microscope, The assay achieved similar to 100 copies/mu L sensitivity in under 30 min of measurement time and accurately detected pre-extracted RNA from a set of positive clinical samples in under 5 min. We combined crRNAs targeting SARS-CoV-2 RNA to improve sensitivity and specificity and directly quantified viral load using enzyme kinetics. Integrated with a reader device based on a mobile phone, this assay has the potential to enable rapid, low-cost, point-of-care screening for SARS-CoV-2. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.title | Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy | - |
dc.type | Article | - |
dc.identifier.wosid | 000612290800009 | - |
dc.identifier.scopusid | 2-s2.0-85097559267 | - |
dc.type.rims | ART | - |
dc.citation.volume | 184 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 323 | - |
dc.citation.endingpage | 333 | - |
dc.citation.publicationname | CELL | - |
dc.identifier.doi | 10.1016/j.cell.2020.12.001 | - |
dc.contributor.localauthor | Son, Sungmin | - |
dc.contributor.nonIdAuthor | Fozouni, Parinaz | - |
dc.contributor.nonIdAuthor | Derby, Maria Diaz de Leon | - |
dc.contributor.nonIdAuthor | Knott, Gavin J. | - |
dc.contributor.nonIdAuthor | Gray, Carley N. | - |
dc.contributor.nonIdAuthor | D'Ambrosio, Michael, V | - |
dc.contributor.nonIdAuthor | Zhao, Chunyu | - |
dc.contributor.nonIdAuthor | Switz, Neil A. | - |
dc.contributor.nonIdAuthor | Kumar, G. Renuka | - |
dc.contributor.nonIdAuthor | Stephens, Stephanie, I | - |
dc.contributor.nonIdAuthor | Boehm, Daniela | - |
dc.contributor.nonIdAuthor | Tsou, Chia-Lin | - |
dc.contributor.nonIdAuthor | Shu, Jeffrey | - |
dc.contributor.nonIdAuthor | Bhuiya, Abdul | - |
dc.contributor.nonIdAuthor | Armstrong, Maxim | - |
dc.contributor.nonIdAuthor | Harris, Andrew R. | - |
dc.contributor.nonIdAuthor | Chen, Pei-Yi | - |
dc.contributor.nonIdAuthor | Osterloh, Jeannette M. | - |
dc.contributor.nonIdAuthor | Meyer-Franke, Anke | - |
dc.contributor.nonIdAuthor | Joehnk, Bastian | - |
dc.contributor.nonIdAuthor | Walcott, Keith | - |
dc.contributor.nonIdAuthor | Sil, Anita | - |
dc.contributor.nonIdAuthor | Langelier, Charles | - |
dc.contributor.nonIdAuthor | Pollard, Katherine S. | - |
dc.contributor.nonIdAuthor | Crawford, Emily D. | - |
dc.contributor.nonIdAuthor | Puschnik, Andreas S. | - |
dc.contributor.nonIdAuthor | Phelps, Maira | - |
dc.contributor.nonIdAuthor | Kistler, Amy | - |
dc.contributor.nonIdAuthor | DeRisi, Joseph L. | - |
dc.contributor.nonIdAuthor | Doudna, Jennifer A. | - |
dc.contributor.nonIdAuthor | Fletcher, Daniel A. | - |
dc.contributor.nonIdAuthor | Ott, Melanie | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | DIAGNOSTIC-TESTS | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordPlus | CAS13B | - |
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