Increased ribosomal protein levels and protein synthesis in the striatal synaptosome of Shank3-overexpressing transgenic mice

Cited 4 time in webofscience Cited 0 time in scopus
  • Hit : 60
  • Download : 0
DC FieldValueLanguage
dc.contributor.authorJin, Chunmeiko
dc.contributor.authorLee, Yeunkumko
dc.contributor.authorKang, Hyojinko
dc.contributor.authorJeong, Kwonko
dc.contributor.authorPark, Jooriko
dc.contributor.authorZhang, Yinhuako
dc.contributor.authorKang, Hyae Rimko
dc.contributor.authorMa, Ruiyingko
dc.contributor.authorSeong, Hyunyoungko
dc.contributor.authorKim, Yoonheeko
dc.contributor.authorJung, Hosungko
dc.contributor.authorKim, Jin Youngko
dc.contributor.authorKim, Yoon Kiko
dc.contributor.authorHan, Kihoonko
dc.identifier.citationMOLECULAR BRAIN, v.14, no.1-
dc.description.abstractThe SH3 and multiple ankyrin repeat domains 3 (Shank3) protein is a core organizer of the macromolecular complex in excitatory postsynapses, and its defects cause numerous synaptopathies, including autism spectrum disorders. Although the function of Shank3 as a postsynaptic scaffold is adequately established, other potential mechanisms through which Shank3 broadly modulates the postsynaptic proteome remain relatively unexplored. In our previous quantitative proteomic analysis, six up-regulated ribosomal proteins were identified in the striatal synaptosome of Shank3-overexpressing transgenic (TG) mice. In the present study, we validated the increased levels of RPLP1 and RPL36A in synaptosome, but not in whole lysate, of the TG striatum. Moreover, protein synthesis and extracellular signaling-regulated kinase (ERK) activity were enhanced in the TG striatal synaptosome. To understand the potential contribution of increased protein synthesis to the proteomic change in the TG striatal synaptosome, we performed RNA-sequencing analyses on both whole synaptosomal and synaptic polysome-enriched fractions. Comparative analyses showed a positive correlation only between the polysome-associated transcriptome and up-regulated proteome in the TG striatal synaptosome. Our findings suggest a novel mechanism through which Shank3 may remodel the postsynaptic proteome by regulating synaptic protein synthesis, whose dysfunction can be implicated in SHANK3-associated synaptopathies.-
dc.titleIncreased ribosomal protein levels and protein synthesis in the striatal synaptosome of Shank3-overexpressing transgenic mice-
dc.citation.publicationnameMOLECULAR BRAIN-
dc.contributor.localauthorKim, Yoon Ki-
dc.contributor.nonIdAuthorJin, Chunmei-
dc.contributor.nonIdAuthorLee, Yeunkum-
dc.contributor.nonIdAuthorKang, Hyojin-
dc.contributor.nonIdAuthorJeong, Kwon-
dc.contributor.nonIdAuthorPark, Joori-
dc.contributor.nonIdAuthorZhang, Yinhua-
dc.contributor.nonIdAuthorKang, Hyae Rim-
dc.contributor.nonIdAuthorMa, Ruiying-
dc.contributor.nonIdAuthorSeong, Hyunyoung-
dc.contributor.nonIdAuthorKim, Yoonhee-
dc.contributor.nonIdAuthorJung, Hosung-
dc.contributor.nonIdAuthorKim, Jin Young-
dc.contributor.nonIdAuthorHan, Kihoon-
dc.subject.keywordAuthorRibosomal protein-
dc.subject.keywordAuthorProtein synthesis-
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 4 items in WoS Click to see citing articles in records_button


  • mendeley


rss_1.0 rss_2.0 atom_1.0