DC Field | Value | Language |
---|---|---|
dc.contributor.author | Isken, Olaf | ko |
dc.contributor.author | Kim, Yoon Ki | ko |
dc.contributor.author | Hosoda, Nao | ko |
dc.contributor.author | Mayeur, Greg L. | ko |
dc.contributor.author | Hershey, John W. B. | ko |
dc.contributor.author | Maquat, Lynne E. | ko |
dc.date.accessioned | 2022-08-04T05:01:07Z | - |
dc.date.available | 2022-08-04T05:01:07Z | - |
dc.date.created | 2022-08-04 | - |
dc.date.created | 2022-08-04 | - |
dc.date.issued | 2008-04 | - |
dc.identifier.citation | CELL, v.133, no.2, pp.314 - 327 | - |
dc.identifier.issn | 0092-8674 | - |
dc.identifier.uri | http://hdl.handle.net/10203/297750 | - |
dc.description.abstract | In mammalian cells, nonsense-mediated mRNA decay (NMD) generally requires that translation terminates sufficiently upstream of a post-splicing exon junction complex (EJC) during a pioneer round of translation. The subsequent binding of Upf1 to the EJC triggers Upf1 phosphorylation. We provide evidence that phospho-Upf1 functions after nonsense codon recognition during steps that involve the translation initiation factor eIF3 and mRNA decay factors. Phospho-Upf1 interacts directly with eIF3 and inhibits the eIF3-dependent conversion of 40S/Met-tRNAi(Met)/mRNA to translationally competent 80S/Met-tRNAi(Met)/mRNA initiation complexes to repress continued translation initiation. Consistent with phospho-Upf1 impairing eIF3 function, NMD fails to detectably target nonsense-containing transcripts that initiate translation independently of eIF3 from the CrPV IRES. There is growing evidence that translational repression is a key transition that precedes mRNA delivery to the degradation machinery. Our results uncover a critical step during NMD that converts a pioneer translation initiation complex to a translationally compromised mRNP. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.title | Upf1 phosphorylation triggers translational repression during nonsense-mediated mRNA decay | - |
dc.type | Article | - |
dc.identifier.wosid | 000255052000020 | - |
dc.identifier.scopusid | 2-s2.0-41949113083 | - |
dc.type.rims | ART | - |
dc.citation.volume | 133 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 314 | - |
dc.citation.endingpage | 327 | - |
dc.citation.publicationname | CELL | - |
dc.identifier.doi | 10.1016/j.cell.2008.02.030 | - |
dc.contributor.localauthor | Kim, Yoon Ki | - |
dc.contributor.nonIdAuthor | Isken, Olaf | - |
dc.contributor.nonIdAuthor | Hosoda, Nao | - |
dc.contributor.nonIdAuthor | Mayeur, Greg L. | - |
dc.contributor.nonIdAuthor | Hershey, John W. B. | - |
dc.contributor.nonIdAuthor | Maquat, Lynne E. | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | EXON JUNCTION COMPLEX | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | SURVEILLANCE COMPLEX | - |
dc.subject.keywordPlus | GENOTOXIC STRESS | - |
dc.subject.keywordPlus | P-BODIES | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | INITIATION | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | YEAST | - |
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