DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Woo-Ri | ko |
dc.contributor.author | Park, Dae-Young | ko |
dc.contributor.author | Kim, Ji Hun | ko |
dc.contributor.author | Lee, Jin-Pyo | ko |
dc.contributor.author | Thai, Nguyen Quang | ko |
dc.contributor.author | Oh, In-Hwan | ko |
dc.contributor.author | Sekhon, Simranjeet Singh | ko |
dc.contributor.author | Choi, Wooil | ko |
dc.contributor.author | Kim, Sung Yeon | ko |
dc.contributor.author | Cho, Byung-Kwan | ko |
dc.contributor.author | Kim, Sun Chang | ko |
dc.contributor.author | Min, Jiho | ko |
dc.contributor.author | Ahn, Ji-Young | ko |
dc.contributor.author | Kim, Yang-Hoon | ko |
dc.date.accessioned | 2022-07-12T02:01:19Z | - |
dc.date.available | 2022-07-12T02:01:19Z | - |
dc.date.created | 2022-07-11 | - |
dc.date.created | 2022-07-11 | - |
dc.date.issued | 2022-04 | - |
dc.identifier.citation | JOURNAL OF NANOBIOTECHNOLOGY, v.20, no.1 | - |
dc.identifier.issn | 1477-3155 | - |
dc.identifier.uri | http://hdl.handle.net/10203/297344 | - |
dc.description.abstract | Background: Glypican-3 (GPC3), a membrane-bound heparan sulfate proteoglycan, is a biomarker of hepatocellular carcinoma (HCC) progression. Aptamers specifically binding to target biomolecules have recently emerged as clinical disease diagnosis targets. Here, we describe 3D structure-based aptaprobe platforms for detecting GPC3, such as aptablotting, aptaprobe-based sandwich assay (ALISA), and aptaprobe-based imaging analysis. Results: For preparing the aptaprobe-GPC3 platforms, we obtained 12 high affinity aptamer candidates (GPC3_1 to GPC3_12) that specifically bind to target GPC3 molecules. Structure-based molecular interactions identified distinct aptatopic residues responsible for binding to the paratopic nucleotide sequences (nt-paratope) of GPC3 aptaprobes. Sandwichable and overlapped aptaprobes were selected through structural analysis. The aptaprobe specificity for using in HCC diagnostics were verified through Aptablotting and ALISA. Moreover, aptaprobe-based imaging showed that the binding property of GPC3_3 and their GPC3 specificity were maintained in HCC xenograft models, which may indicate a new HCC imaging diagnosis. Conclusion: Aptaprobe has the potential to be used as an affinity reagent to detect the target in vivo and in vitro diagnosing system. | - |
dc.language | English | - |
dc.publisher | BMC | - |
dc.title | Structure based innovative approach to analyze aptaprobe-GPC3 complexes in hepatocellular carcinoma | - |
dc.type | Article | - |
dc.identifier.wosid | 000818737800001 | - |
dc.identifier.scopusid | 2-s2.0-85128890461 | - |
dc.type.rims | ART | - |
dc.citation.volume | 20 | - |
dc.citation.issue | 1 | - |
dc.citation.publicationname | JOURNAL OF NANOBIOTECHNOLOGY | - |
dc.identifier.doi | 10.1186/s12951-022-01391-z | - |
dc.contributor.localauthor | Cho, Byung-Kwan | - |
dc.contributor.localauthor | Kim, Sun Chang | - |
dc.contributor.nonIdAuthor | Shin, Woo-Ri | - |
dc.contributor.nonIdAuthor | Park, Dae-Young | - |
dc.contributor.nonIdAuthor | Lee, Jin-Pyo | - |
dc.contributor.nonIdAuthor | Thai, Nguyen Quang | - |
dc.contributor.nonIdAuthor | Oh, In-Hwan | - |
dc.contributor.nonIdAuthor | Sekhon, Simranjeet Singh | - |
dc.contributor.nonIdAuthor | Choi, Wooil | - |
dc.contributor.nonIdAuthor | Kim, Sung Yeon | - |
dc.contributor.nonIdAuthor | Min, Jiho | - |
dc.contributor.nonIdAuthor | Ahn, Ji-Young | - |
dc.contributor.nonIdAuthor | Kim, Yang-Hoon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Glypican-3 | - |
dc.subject.keywordAuthor | Hepatocellular carcinoma | - |
dc.subject.keywordAuthor | Cancer diagnosis | - |
dc.subject.keywordAuthor | Aptaprobe | - |
dc.subject.keywordAuthor | Structure-based molecular interaction | - |
dc.subject.keywordAuthor | Aptablotting | - |
dc.subject.keywordAuthor | ALISA | - |
dc.subject.keywordAuthor | Aptaprobe-based imaging | - |
dc.subject.keywordPlus | GLYPICAN-3 | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | SERUM | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | BIODISTRIBUTION | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | MARKER | - |
dc.subject.keywordPlus | CANCER | - |
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