DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Lee, Sang-Yup | - |
dc.contributor.advisor | 이상엽 | - |
dc.contributor.author | Lee, Seung-Hwan | - |
dc.contributor.author | 이승환 | - |
dc.date.accessioned | 2011-12-13T01:51:31Z | - |
dc.date.available | 2011-12-13T01:51:31Z | - |
dc.date.issued | 2000 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=158573&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/29673 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 화학공학과, 2000.2, [ iv, 43 p. ] | - |
dc.description.abstract | High cell density fed-batch cultures of Pseudomonas putida were carried out for the production of medium-chain-length polyhydroxyalkanoates (PHAs) using oleic acid as a carbon source. By employing an optimal feeding strategy without the limitation of any nutrient, high cell concentration of 173 g/L was achieved, but the PHA concentration and PHA content were only 32.3 g/L and 18.7 wt%. To increase the PHA concentration and PHA content, phosphorus limitation was applied during the fed-bath culture by reducing the initial $KH_2PO_4$ concentration. When the initial $KH_2PO_4$ concentration was reduced to 4 g/L, cell concentration, PHA concentration and PHA content obtained in 38 h were 141 g/L, 72.6 g/L and 51.4 wt%, respectively, resulting in a high productivity of 1.91 g PHA/L-h. Newly screened Pseudomonas sp. KR-22 from soil sample produced MCL-PHA mainly composed of 3-hydroxy hexanoic acid, 3-hydroxy octanoic acid, 3- hydroxy decanoic acid, 3-hydroxy dodecanoic acid, and 3-hydroxy-5-cis-tetradecenoic acid. By fed-batch culture, 82 g/L of DCW and 28% of PHA content was obtained in 41 h. This resulted in a productivity of 0.56 g/L-h. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Medium-chain-length | - |
dc.subject | Polyhydroxyalkanoate | - |
dc.subject | Fed-batch | - |
dc.subject | 유가식 배양 | - |
dc.subject | 생분해성 고분자 | - |
dc.subject | Pseudomonas | - |
dc.title | Development of fed-batch culture for the production of Medium-chain-length polyhydroxyalkanoate by pseudomonas | - |
dc.title.alternative | Medium-chain-length Polyhydroxyalkanoate 고농도 생산을 위한 Pseudomonas 균주의 유가식 배양기술 개발 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 158573/325007 | - |
dc.description.department | 한국과학기술원 : 화학공학과, | - |
dc.identifier.uid | 000983427 | - |
dc.contributor.localauthor | Lee, Sang-Yup | - |
dc.contributor.localauthor | 이상엽 | - |
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