DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sonawane, Prasad M. | ko |
dc.contributor.author | Lee, Woohyun | ko |
dc.contributor.author | Kim, Yunsu | ko |
dc.contributor.author | Roychaudhury, Arkaprava | ko |
dc.contributor.author | Bhosale, Vikas K. | ko |
dc.contributor.author | Kim, Donghyeon | ko |
dc.contributor.author | Park, Hee-Sung | ko |
dc.contributor.author | Kim, Cheol-Hee | ko |
dc.contributor.author | Churchill, David G. | ko |
dc.date.accessioned | 2022-05-09T03:01:53Z | - |
dc.date.available | 2022-05-09T03:01:53Z | - |
dc.date.created | 2022-05-09 | - |
dc.date.created | 2022-05-09 | - |
dc.date.created | 2022-05-09 | - |
dc.date.issued | 2022-02 | - |
dc.identifier.citation | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, v.267 | - |
dc.identifier.issn | 1386-1425 | - |
dc.identifier.uri | http://hdl.handle.net/10203/296461 | - |
dc.description.abstract | Potent oxidants such as peroxynitrite (ONOO-) play important roles in the regulation of different physiopathological processes; their overproduction is thought to potentially cause several diseases in living organisms. Hence, the precise and selective monitoring of ONOO- is imperative for elucidating its interplay and roles in pathological and physiological processes. Herein, we present a novel diphenyl phosphinate-masked benzoindocyanin "turn-on " fluorogenic probe to help detect mitochondrial ONOO- in living cells and zebrafish models. A pale yellow color solution of BICBzDP turns rose-red upon the addition of ONOO- , selectively, contrary to that of other competitive bioactive molecules. BICBzDP displays an ultra-sensitivity detection limit (47.8 nM) with outstanding selectivity and sensitivity towards mitochondrial ONOO- and possesses a notable 68-fold fluorescence enhancement involving a large redshift of 91 nm. Importantly, further biological experimental investigations with BICBzDP indicate specific sensitivity and reliability of the probe to track the ONOO- level, not only in live cells, but also demonstrates dynamic fluctuations in the inflammatory zebrafish animal models. Thus, BICBzDP could be employed as a future potential biological tool for exploiting the role of ONOO- in a variety of different physiological systems.(C) 2021 Published by Elsevier B.V. | - |
dc.language | English | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.title | Phosphinate-benzoindocyanin fluorescent probe for endogenous mitochondrial peroxynitrite detection in living cells and gallbladder access in inflammatory zebrafish animal models | - |
dc.type | Article | - |
dc.identifier.wosid | 000786604400019 | - |
dc.identifier.scopusid | 2-s2.0-85119047241 | - |
dc.type.rims | ART | - |
dc.citation.volume | 267 | - |
dc.citation.publicationname | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | - |
dc.identifier.doi | 10.1016/j.saa.2021.120568 | - |
dc.contributor.localauthor | Park, Hee-Sung | - |
dc.contributor.localauthor | Churchill, David G. | - |
dc.contributor.nonIdAuthor | Roychaudhury, Arkaprava | - |
dc.contributor.nonIdAuthor | Bhosale, Vikas K. | - |
dc.contributor.nonIdAuthor | Kim, Cheol-Hee | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Mitochondria-accessing | - |
dc.subject.keywordAuthor | Diphenyl phosphinate | - |
dc.subject.keywordAuthor | ONOO- | - |
dc.subject.keywordAuthor | Cell imaging | - |
dc.subject.keywordAuthor | Zebrafish | - |
dc.subject.keywordPlus | TURN-ON PROBE | - |
dc.subject.keywordPlus | SELECTIVE DETECTION | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.subject.keywordPlus | BIOCHEMISTRY | - |
dc.subject.keywordPlus | CYSTEINE | - |
dc.subject.keywordPlus | SHIFT | - |
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