Directed evolution of Chlorella sp. HS2 towards enhanced lipid accumulation by ethyl methanesulfonate mutagenesis in conjunction with fluorescence-activated cell sorting based screening

Cited 9 time in webofscience Cited 0 time in scopus
  • Hit : 224
  • Download : 0
DC FieldValueLanguage
dc.contributor.authorNayak, Manoranjanko
dc.contributor.authorSuh, Williamko
dc.contributor.authorOh, Young Taekko
dc.contributor.authorRyu, Ae Jinko
dc.contributor.authorJeong, Ki Junko
dc.contributor.authorKim, Minsikko
dc.contributor.authorMohapatra, Ranjan Kumarko
dc.contributor.authorLee, Bongsooko
dc.contributor.authorChang, Yong Keunko
dc.date.accessioned2022-05-06T05:00:40Z-
dc.date.available2022-05-06T05:00:40Z-
dc.date.created2022-05-06-
dc.date.created2022-05-06-
dc.date.issued2022-05-
dc.identifier.citationFUEL, v.316-
dc.identifier.issn0016-2361-
dc.identifier.urihttp://hdl.handle.net/10203/296356-
dc.description.abstractThe present study aimed to improve the lipid content and lipid productivity of Chlorella sp. HS2 by exposing them to ethyl methanesulfonate (EMS) to generate a mutant and followed with fluorescent assorted cell sorting (FACS) for the selection of mutant cells having desirable phenotypes. The microalgae were subjected under 100 mu mol mL(-1) concentration of EMS for 30 min, followed by intracellular lipid staining with boron-dipyrromethene (BODIPY) fluorophore for FACS based selection. The biomass growth, lipid content, lipid productivity, and fatty acid composition of the selected mutagenized strain (SMS, TE+F) of Chlorella sp. HS2 were compared against those of FACS selected strain without mutagenesis (FSS, T-F) and parent wild strain (PWS, TC), under two different carbon supplementation schemes (only CO2 supply and optimized carbon supply). The highest lipid content and productivity of 35.6% and 248.7 mg L-1 d-(1), respectively were observed in the SMS grown under optimized carbon supplementation (1% CO2 (v/v) and 0.5 g L-1 NaHCO3), which was a substantial improvement over those of FSS (26.6% and 193.4 mg L(-1 )d(-1)) and PWS (24.7% and 153.6 mg L-1 d(-1)). The fatty acid methyl ester (FAME) profile of the lipids derived from SMS showed a marked increase in the proportion of saturated fatty acids (SFA) and mono-unsaturated fatty acids (MUFA) (76.7%) over those of FSS (70.2%) and PWS (69.6%). Lastly, fuel properties were found to be appropriate for biodiesel production, as they corroborate with international fuel standards. These results indicate that directed evolution using EMS mutagenesis with FACS is a powerful tool to achieve microalgal strain improvement for biofuel production.-
dc.languageEnglish-
dc.publisherELSEVIER SCI LTD-
dc.titleDirected evolution of Chlorella sp. HS2 towards enhanced lipid accumulation by ethyl methanesulfonate mutagenesis in conjunction with fluorescence-activated cell sorting based screening-
dc.typeArticle-
dc.identifier.wosid000783173000010-
dc.identifier.scopusid2-s2.0-85123592618-
dc.type.rimsART-
dc.citation.volume316-
dc.citation.publicationnameFUEL-
dc.identifier.doi10.1016/j.fuel.2022.123410-
dc.contributor.localauthorJeong, Ki Jun-
dc.contributor.localauthorChang, Yong Keun-
dc.contributor.nonIdAuthorNayak, Manoranjan-
dc.contributor.nonIdAuthorSuh, William-
dc.contributor.nonIdAuthorOh, Young Taek-
dc.contributor.nonIdAuthorMohapatra, Ranjan Kumar-
dc.contributor.nonIdAuthorLee, Bongsoo-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorChlorella sp-
dc.subject.keywordAuthorHS2-
dc.subject.keywordAuthorEMS-
dc.subject.keywordAuthorFACS-
dc.subject.keywordAuthorLipid productivity-
dc.subject.keywordAuthorFAME-
dc.subject.keywordAuthorBiodiesel-
dc.subject.keywordPlusPRODUCTIVITY-
dc.subject.keywordPlusMICROALGAE-
dc.subject.keywordPlusBICARBONATE-
dc.subject.keywordPlusBIOMASS-
dc.subject.keywordPlusEMS-
dc.subject.keywordPlusSUPPLEMENTATION-
dc.subject.keywordPlusBIODIESEL-
dc.subject.keywordPlusVULGARIS-
dc.subject.keywordPlusPROFILE-
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 9 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0