Production of short-chain-length and medium-chain-length polyhydroxyalkanoate copolymer through systems metabolic engineering시스템 대사 공학을 통한 짧은사슬길이와 중간사슬길이 폴리하이드록시알칸산 공중합체 생산

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Since there are severe global climate change and depletion of petroleum-based resources, it is essential to find sustainable alternative material replacing conventional plastic material based on petrochemical industries. Polyhydroxyalkanoate is promising substitute material with the advantages of biodegradability. Poly(3-hydroxybutyrate-co-lactate) is the microbial polyester that can improve poor material properties of poly(3-hydroxybutyrate) with an additional incorporation of lactate. In this study, the different expressions of propionyl-CoA transferase mutant and PHA synthase mutant were introduced in metabolically engineered Escherichia coli, and optimal production system showed 125.9 g/L of P(3HB-co-5.9mol% LA). Moreover, the production of polyester containing medium-chain-length monomer from fatty acid or glucose was discussed for enhanced material properties. Furthermore, the extraction of PHA granules based on alkaline hydrolysis and analysis of material properties were examined.
Advisors
Lee, Sang Yupresearcher이상엽researcher
Description
한국과학기술원 :생명화학공학과,
Publisher
한국과학기술원
Issue Date
2021
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2021.2,[v, 51 p. :]

Keywords

Polyhydroxyalkanoate▼aEscherichia coli▼aMetabolic engineering▼aLactate▼aFatty acid biosynthesis; 폴리하이드록시알칸산▼a대장균▼a대사 공학▼a락테이트▼a지방산 생합성

URI
http://hdl.handle.net/10203/295378
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=957329&flag=dissertation
Appears in Collection
CBE-Theses_Master(석사논문)
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