DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Park, Je-Kyun | - |
dc.contributor.advisor | 박제균 | - |
dc.contributor.author | Roh, Hyewon | - |
dc.date.accessioned | 2022-04-21T19:30:56Z | - |
dc.date.available | 2022-04-21T19:30:56Z | - |
dc.date.issued | 2021 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=948581&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/295270 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2021.2,[vii, 57 p. :] | - |
dc.description.abstract | Spheroid, a 3D aggregate of tumor cells in a spherical shape, has overcome the limitations of conventional 3D cell models to accurately mimic the in-vivo environment of a human body. The spheroids are often cultured with other primary cells and embedded in collagen drops to construct a spheroid–hydrogel model that better mimics the cell–cell and cell–extracellular matrix (ECM) interactions. However, the conventionally used methods of culturing and embedding spheroids into ECM has several shortcomings. The procedure of transferring a single spheroid at a time by manual pipetting results in the inconsistent arrangement and even loss or damage of the spheroid. Based on the previously introduced droplet contact-based spheroid transfer technique, we present a poly(dimethylsiloxane) and resin-based drop array chip and pillar array chip with alignment stoppers, which enhances the alignment between the chips for uniform placement of spheroids and eliminates the need to use a stereomicroscope while handling the cell models. The novel platform allows a homogeneous and time-efficient construction and diverse analysis of an array of fibroblast-associated glioblastoma multiforme spheroids embedded in collagen. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 3D cell culture | - |
dc.subject | Droplet-based contact transfer | - |
dc.subject | Drug assay model | - |
dc.subject | Fibroblast | - |
dc.subject | Glioblastoma multiforme | - |
dc.subject | Hanging drop culture | - |
dc.subject | Spheroid invasion assay | - |
dc.subject | 3차원 세포 배양 | - |
dc.subject | 뇌종양 | - |
dc.subject | 섬유아세포 | - |
dc.subject | 스페로이드 침투 분석 | - |
dc.subject | 약물 평가 모델 | - |
dc.subject | 액적 접촉 기반 이식 | - |
dc.subject | 현적 배양 | - |
dc.title | Construction of a fibroblast-associated tumor spheroid model based on collagen drop array chip | - |
dc.title.alternative | 콜라겐 액적 어레이 칩에 기반한 섬유아세포가 포함된 종양 스페로이드 모델의 구축 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :바이오및뇌공학과, | - |
dc.contributor.alternativeauthor | 노혜원 | - |
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