DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Lee, Daeyoup | - |
dc.contributor.advisor | 이대엽 | - |
dc.contributor.author | Cheon, Youngseo | - |
dc.date.accessioned | 2022-04-15T01:54:10Z | - |
dc.date.available | 2022-04-15T01:54:10Z | - |
dc.date.issued | 2021 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=962394&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/294622 | - |
dc.description.abstract | Promoter-proximal pausing of RNA polymerase Ⅱ (RNAPⅡ) is a critical step for the precise regulation of gene expression. Here, we provide evidence of promoter-proximal pausing-like distributions of RNAPⅡ in S. cerevisiae using PRO-seq experiments. Furthermore, we find that knockdown of the chromatin remodeler Ino80p causes the transition of RNAPⅡ pausing from the main pausing site to the alternative pausing site. In addition, these Ino80p-dependent genes exhibit the +1 nucleosome closely associated with the alternative pausing site, which implied the suppression of the alternative pausing site in a nucleosome context-dependent manner. Ino80p knockdown results in the increase of nucleosome fuzziness and the decrease of nucleosome stability for these genes, suggesting the role of the Ino80 complex to regulate the +1 nucleosome is highly correlated with the proper localization of RNAPⅡ pausing. Moreover, we observed similar INO80-dependent RNAPⅡ pausing site determination in mouse embryonic stem cells (mESCs). Based on our collective results, we hypothesize that the chromatin remodeler Ino80p plays a highly conserved role in regulating early RNAPⅡ elongation to establish intact pausing in various organisms, from budding yeast to mouse. | - |
dc.language | eng | - |
dc.title | Mechanistic study of nucleosome dynamics and transcriptional regulation by the Ino80 complex | - |
dc.title.alternative | Ino80 복합체에 의한 뉴클레오좀 다이나믹스 및 전사 조절 기전에 관한 연구 | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :생명과학과, | - |
dc.description.isOpenAccess | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2021.8,[vi, 125 p. :] | - |
dc.publisher.country | 한국과학기술원 | - |
dc.type.journalArticle | Thesis(Ph.D) | - |
dc.contributor.alternativeauthor | 천영서 | - |
dc.subject.keywordAuthor | The chromatin remodeler Ino80p▼apromoter-proximal RNAPⅡ pausing▼aPRO-seq▼aalternative pausing site▼a+1 nucleosome▼aAID system | - |
dc.subject.keywordAuthor | 염색질 개조 효소 Ino80▼a프로모터 근접 부위 RNA 중합효소 Ⅱ 멈춤▼aPRO-seq▼a대체 멈춤 부위▼a+1 뉴클레오좀▼aAID 시스템 | - |
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