DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Ryanggeun | ko |
dc.contributor.author | Kang, Moo-Koo | ko |
dc.contributor.author | Kim, Yong-Jin | ko |
dc.contributor.author | Yang, Bobae | ko |
dc.contributor.author | Shim, Hwanyong | ko |
dc.contributor.author | Kim, Sugyung | ko |
dc.contributor.author | Kim, Kyungwoo | ko |
dc.contributor.author | Yang, Chul Min | ko |
dc.contributor.author | Min, Byeong-gyu | ko |
dc.contributor.author | Jung, Woong-Jae | ko |
dc.contributor.author | Lee, Eun-Chong | ko |
dc.contributor.author | Joo, Jung-Sik | ko |
dc.contributor.author | Park, Gunhee | ko |
dc.contributor.author | Cho, Won-Ki | ko |
dc.contributor.author | Kim, Hyoung-Pyo | ko |
dc.date.accessioned | 2022-01-20T06:40:56Z | - |
dc.date.available | 2022-01-20T06:40:56Z | - |
dc.date.created | 2022-01-15 | - |
dc.date.created | 2022-01-15 | - |
dc.date.created | 2022-01-15 | - |
dc.date.created | 2022-01-15 | - |
dc.date.issued | 2022-01 | - |
dc.identifier.citation | NUCLEIC ACIDS RESEARCH, v.50, no.1, pp.207 - 226 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | http://hdl.handle.net/10203/291902 | - |
dc.description.abstract | CTCF is crucial to the organization of mammalian genomes into loop structures. According to recent studies, the transcription apparatus is compartmentalized and concentrated at super-enhancers to form phase-separated condensates and drive the expression of cell-identity genes. However, it remains unclear whether and how transcriptional condensates are coupled to higher-order chromatin organization. Here, we show that CTCF is essential for RNA polymerase II (Pol II)-mediated chromatin interactions, which occur as hyperconnected spatial clusters at super-enhancers. We also demonstrate that CTCF clustering, unlike Pol II clustering, is independent of liquid-liquid phase-separation and resistant to perturbation of transcription. Interestingly, clusters of Pol II, BRD4, and MED1 were found to dissolve upon CTCF depletion, but were reinstated upon restoration of CTCF, suggesting a potent instructive function for CTCF in the formation of transcriptional condensates. Overall, we provide evidence suggesting that CTCF-mediated chromatin looping acts as an architectural prerequisite for the assembly of phase-separated transcriptional condensates. | - |
dc.language | English | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | CTCF-mediated chromatin looping provides a topological framework for the formation of phase-separated transcriptional condensates | - |
dc.type | Article | - |
dc.identifier.wosid | 000749588900020 | - |
dc.identifier.scopusid | 2-s2.0-85123651075 | - |
dc.type.rims | ART | - |
dc.citation.volume | 50 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 207 | - |
dc.citation.endingpage | 226 | - |
dc.citation.publicationname | NUCLEIC ACIDS RESEARCH | - |
dc.identifier.doi | 10.1093/nar/gkab1242 | - |
dc.contributor.localauthor | Cho, Won-Ki | - |
dc.contributor.nonIdAuthor | Lee, Ryanggeun | - |
dc.contributor.nonIdAuthor | Kang, Moo-Koo | - |
dc.contributor.nonIdAuthor | Kim, Yong-Jin | - |
dc.contributor.nonIdAuthor | Yang, Bobae | - |
dc.contributor.nonIdAuthor | Kim, Sugyung | - |
dc.contributor.nonIdAuthor | Kim, Kyungwoo | - |
dc.contributor.nonIdAuthor | Yang, Chul Min | - |
dc.contributor.nonIdAuthor | Min, Byeong-gyu | - |
dc.contributor.nonIdAuthor | Jung, Woong-Jae | - |
dc.contributor.nonIdAuthor | Lee, Eun-Chong | - |
dc.contributor.nonIdAuthor | Joo, Jung-Sik | - |
dc.contributor.nonIdAuthor | Park, Gunhee | - |
dc.contributor.nonIdAuthor | Kim, Hyoung-Pyo | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | GLOBIN LOCUS REQUIRESCELL IDENTITYSUPER-ENHANCERSGENE-EXPRESSIONREAD ALIGNMENTGENOMECOHESINDOMAINSORGANIZATIONBINDING | - |
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