Production of the Minor Ginsenoside F-2 from the PPD-mix-type Major Ginsenosides Using a Novel Recombinant Glycoside Hydrolase from Novosphingobium aromaticivorans

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dc.contributor.authorSiddiqi, Muhammad Zubairko
dc.contributor.authorPark, Hye Yoonko
dc.contributor.authorKim, Ga-Ryunko
dc.contributor.authorCui, Chang-Haoko
dc.contributor.authorJo, Young Junko
dc.contributor.authorKim, Sun-Changko
dc.contributor.authorIm, Wan-Taekko
dc.date.accessioned2022-01-11T06:43:50Z-
dc.date.available2022-01-11T06:43:50Z-
dc.date.created2021-12-07-
dc.date.created2021-12-07-
dc.date.created2021-12-07-
dc.date.issued2021-12-
dc.identifier.citationBIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.26, no.6, pp.956 - 967-
dc.identifier.issn1226-8372-
dc.identifier.urihttp://hdl.handle.net/10203/291731-
dc.description.abstractBackground: The minor ginsenosides such as F-2, C-K, Rh2, and Rg3 make up less than 1% of the total ginseng extract; however, many studies have shown that ginsenoside F-2 has anti-cancer and antioxidant effects and improves dementia and atopic dermatitis. Therefore, the enhanced production of this minor ginsenoside is a promising approach for the pharmaceutical industry. In this study, we found and cloned a novel glycoside hydrolase gene for the gram-scale production of ginsenoside F-2. Methods and Results: Novosphingobium aromaticivorans, which was isolated from deep-terrestrial-subsurface sediments, has shown ginsenoside-converting ability. From this bacterium, a novel glycoside hydrolase, named BglNar, was found that can efficiently biotransform the protopanaxatriol (PPD-mix-type) major ginsenosides (Rb-1, Rb-2 Rc, and Rd) into the minor ginsenoside F-2. This enzyme was cloned, expressed in Escherichia coli BL21, and characterized. The BglNar comprises 439 amino acid and belongs to the glycoside hydrolase family 1. The K-m value of p-nitrophenyl-beta-D-glucopyranoside was 9.06 +/- 0.28 and the V-max value was 24.0 +/- 0.34 mu mol/min/mg of protein. For gram-scale production of minor ginsenoside F-2, crude PPD-mix-type ginsenosides (2 g/400 mL) were treated with BglNar and 1.14 g of F-2 with final purity of 82.5 +/- 1.3%, was obtained after purification using a column packed with HP20 resin. Conclusion: Our preliminary data demonstrate that the gram production of ginsenoside F-2 using a recombinant enzyme will enhance the health benefits of Panax ginseng. This is the first study describing the gram-scale production of F-2 from PPD-mix using a single novel ginsenoside-transforming beta-glucosidase of the GH family 1. Significance and Impact of Study: In most of the previous studies, researchers have been used the combination of enzymes for production of F-2, while in this study we found the possibility of gram-scale production of F-2 from PPD-mix-type major ginsenosides using a single recombinant enzyme, thus simplifying the production and reducing the cost.-
dc.languageEnglish-
dc.publisherKOREAN SOC BIOTECHNOLOGY & BIOENGINEERING-
dc.titleProduction of the Minor Ginsenoside F-2 from the PPD-mix-type Major Ginsenosides Using a Novel Recombinant Glycoside Hydrolase from Novosphingobium aromaticivorans-
dc.typeArticle-
dc.identifier.wosid000722487800001-
dc.identifier.scopusid2-s2.0-85119877323-
dc.type.rimsART-
dc.citation.volume26-
dc.citation.issue6-
dc.citation.beginningpage956-
dc.citation.endingpage967-
dc.citation.publicationnameBIOTECHNOLOGY AND BIOPROCESS ENGINEERING-
dc.identifier.doi10.1007/s12257-020-0215-2-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.identifier.kciidART002800615-
dc.contributor.localauthorKim, Sun-Chang-
dc.contributor.nonIdAuthorSiddiqi, Muhammad Zubair-
dc.contributor.nonIdAuthorPark, Hye Yoon-
dc.contributor.nonIdAuthorKim, Ga-Ryun-
dc.contributor.nonIdAuthorIm, Wan-Taek-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorbiotransformation-
dc.subject.keywordAuthorbeta-glucosidase-
dc.subject.keywordAuthorrecombinant enzyme-
dc.subject.keywordAuthorginsenoside F-2-
dc.subject.keywordAuthorNovosphingobium aromaticivorans-
dc.subject.keywordPlusTHERMOSTABLE BETA-GLYCOSIDASE-
dc.subject.keywordPlusSP NOV.-
dc.subject.keywordPlusCONVERTING ACTIVITY-
dc.subject.keywordPlusENHANCED PRODUCTION-
dc.subject.keywordPlusCOMPOUND K-
dc.subject.keywordPlusGEN. NOV.-
dc.subject.keywordPlusGLUCOSIDASE-
dc.subject.keywordPlusF2-
dc.subject.keywordPlusRB-1-
dc.subject.keywordPlusCLASSIFICATION-
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