DC Field | Value | Language |
---|---|---|
dc.contributor.author | Siddiqi, Muhammad Zubair | ko |
dc.contributor.author | Park, Hye Yoon | ko |
dc.contributor.author | Kim, Ga-Ryun | ko |
dc.contributor.author | Cui, Chang-Hao | ko |
dc.contributor.author | Jo, Young Jun | ko |
dc.contributor.author | Kim, Sun-Chang | ko |
dc.contributor.author | Im, Wan-Taek | ko |
dc.date.accessioned | 2022-01-11T06:43:50Z | - |
dc.date.available | 2022-01-11T06:43:50Z | - |
dc.date.created | 2021-12-07 | - |
dc.date.created | 2021-12-07 | - |
dc.date.created | 2021-12-07 | - |
dc.date.issued | 2021-12 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.26, no.6, pp.956 - 967 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | http://hdl.handle.net/10203/291731 | - |
dc.description.abstract | Background: The minor ginsenosides such as F-2, C-K, Rh2, and Rg3 make up less than 1% of the total ginseng extract; however, many studies have shown that ginsenoside F-2 has anti-cancer and antioxidant effects and improves dementia and atopic dermatitis. Therefore, the enhanced production of this minor ginsenoside is a promising approach for the pharmaceutical industry. In this study, we found and cloned a novel glycoside hydrolase gene for the gram-scale production of ginsenoside F-2. Methods and Results: Novosphingobium aromaticivorans, which was isolated from deep-terrestrial-subsurface sediments, has shown ginsenoside-converting ability. From this bacterium, a novel glycoside hydrolase, named BglNar, was found that can efficiently biotransform the protopanaxatriol (PPD-mix-type) major ginsenosides (Rb-1, Rb-2 Rc, and Rd) into the minor ginsenoside F-2. This enzyme was cloned, expressed in Escherichia coli BL21, and characterized. The BglNar comprises 439 amino acid and belongs to the glycoside hydrolase family 1. The K-m value of p-nitrophenyl-beta-D-glucopyranoside was 9.06 +/- 0.28 and the V-max value was 24.0 +/- 0.34 mu mol/min/mg of protein. For gram-scale production of minor ginsenoside F-2, crude PPD-mix-type ginsenosides (2 g/400 mL) were treated with BglNar and 1.14 g of F-2 with final purity of 82.5 +/- 1.3%, was obtained after purification using a column packed with HP20 resin. Conclusion: Our preliminary data demonstrate that the gram production of ginsenoside F-2 using a recombinant enzyme will enhance the health benefits of Panax ginseng. This is the first study describing the gram-scale production of F-2 from PPD-mix using a single novel ginsenoside-transforming beta-glucosidase of the GH family 1. Significance and Impact of Study: In most of the previous studies, researchers have been used the combination of enzymes for production of F-2, while in this study we found the possibility of gram-scale production of F-2 from PPD-mix-type major ginsenosides using a single recombinant enzyme, thus simplifying the production and reducing the cost. | - |
dc.language | English | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.title | Production of the Minor Ginsenoside F-2 from the PPD-mix-type Major Ginsenosides Using a Novel Recombinant Glycoside Hydrolase from Novosphingobium aromaticivorans | - |
dc.type | Article | - |
dc.identifier.wosid | 000722487800001 | - |
dc.identifier.scopusid | 2-s2.0-85119877323 | - |
dc.type.rims | ART | - |
dc.citation.volume | 26 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 956 | - |
dc.citation.endingpage | 967 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.identifier.doi | 10.1007/s12257-020-0215-2 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.identifier.kciid | ART002800615 | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.nonIdAuthor | Siddiqi, Muhammad Zubair | - |
dc.contributor.nonIdAuthor | Park, Hye Yoon | - |
dc.contributor.nonIdAuthor | Kim, Ga-Ryun | - |
dc.contributor.nonIdAuthor | Im, Wan-Taek | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | biotransformation | - |
dc.subject.keywordAuthor | beta-glucosidase | - |
dc.subject.keywordAuthor | recombinant enzyme | - |
dc.subject.keywordAuthor | ginsenoside F-2 | - |
dc.subject.keywordAuthor | Novosphingobium aromaticivorans | - |
dc.subject.keywordPlus | THERMOSTABLE BETA-GLYCOSIDASE | - |
dc.subject.keywordPlus | SP NOV. | - |
dc.subject.keywordPlus | CONVERTING ACTIVITY | - |
dc.subject.keywordPlus | ENHANCED PRODUCTION | - |
dc.subject.keywordPlus | COMPOUND K | - |
dc.subject.keywordPlus | GEN. NOV. | - |
dc.subject.keywordPlus | GLUCOSIDASE | - |
dc.subject.keywordPlus | F2 | - |
dc.subject.keywordPlus | RB-1 | - |
dc.subject.keywordPlus | CLASSIFICATION | - |
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