DC Field | Value | Language |
---|---|---|
dc.contributor.author | Boonruangkan, Jeeranan | ko |
dc.contributor.author | Farrokhi, Hamid | ko |
dc.contributor.author | Rohith, Thazhe M. | ko |
dc.contributor.author | Kwok, Samuel | ko |
dc.contributor.author | Carney, Tom J. | ko |
dc.contributor.author | Su, Pei-Chen | ko |
dc.contributor.author | Kim, Young-Jin | ko |
dc.date.accessioned | 2021-12-14T06:42:37Z | - |
dc.date.available | 2021-12-14T06:42:37Z | - |
dc.date.created | 2021-12-14 | - |
dc.date.created | 2021-12-14 | - |
dc.date.created | 2021-12-14 | - |
dc.date.issued | 2021-11 | - |
dc.identifier.citation | JOURNAL OF BIOMEDICAL OPTICS, v.26, no.11 | - |
dc.identifier.issn | 1083-3668 | - |
dc.identifier.uri | http://hdl.handle.net/10203/290537 | - |
dc.description.abstract | Significance: Real-time monitoring of the heart rate and blood flow is crucial for studying cardiovascular dysfunction, which leads to cardiovascular diseases. Aim: This study aims at in-depth understanding of high-speed cardiovascular dynamics in a zebrafish embryo model for various biomedical applications via frequency-comb-referenced quantitative phase imaging (FCR-QPI). Approach: Quantitative phase imaging (QPI) has emerged as a powerful technique in the field of biomedicine but has not been actively applied to the monitoring of circulatory/cardiovascular parameters, due to dynamic speckles and low frame rates. We demonstrate FCR-QPI to measure heart rate and blood flow in a zebrafish embryo. FCR-QPI utilizes a high-speed photodetector instead of a conventional camera, so it enables real-time monitoring of individual red blood cell (RBC) flow. Results: The average velocity of zebrafish' s RBCs was measured from 192.5 to 608.8 mu m/s at 24 to 28 hour-post-fertilization (hpf). In addition, the number of RBCs in a pulsatile blood flow was revealed to 16 cells/pulse at 48 hpf. The heart rates corresponded to 94 and 142 beats-per-minute at 24 and 48 hpf. Conclusions: This approach will newly enable in-depth understanding of the cardiovascular dynamics in the zebrafish model and possible usage for drug discovery applications in biomedicine. (C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. | - |
dc.language | English | - |
dc.publisher | SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS | - |
dc.title | Label-free quantitative measurement of cardiovascular dynamics in a zebrafish embryo using frequency-comb-referenced-quantitative phase imaging | - |
dc.type | Article | - |
dc.identifier.wosid | 000727200100004 | - |
dc.identifier.scopusid | 2-s2.0-85120864091 | - |
dc.type.rims | ART | - |
dc.citation.volume | 26 | - |
dc.citation.issue | 11 | - |
dc.citation.publicationname | JOURNAL OF BIOMEDICAL OPTICS | - |
dc.identifier.doi | 10.1117/1.JBO.26.11.116004 | - |
dc.contributor.localauthor | Kim, Young-Jin | - |
dc.contributor.nonIdAuthor | Boonruangkan, Jeeranan | - |
dc.contributor.nonIdAuthor | Farrokhi, Hamid | - |
dc.contributor.nonIdAuthor | Rohith, Thazhe M. | - |
dc.contributor.nonIdAuthor | Kwok, Samuel | - |
dc.contributor.nonIdAuthor | Carney, Tom J. | - |
dc.contributor.nonIdAuthor | Su, Pei-Chen | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | quantitative phase imaging | - |
dc.subject.keywordAuthor | frequency comb | - |
dc.subject.keywordAuthor | cardiovascular dynamics | - |
dc.subject.keywordAuthor | zebrafish | - |
dc.subject.keywordAuthor | high-speed phase measurement | - |
dc.subject.keywordPlus | BLOOD-FLOW | - |
dc.subject.keywordPlus | ABNORMALITIES | - |
dc.subject.keywordPlus | DYSFUNCTION | - |
dc.subject.keywordPlus | DROSOPHILA | - |
dc.subject.keywordPlus | EVENTS | - |
dc.subject.keywordPlus | MODEL | - |
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