Target-induced transcription of a light-up RNA aptamer to construct a novel method for alkaline phosphatase assay
We herein developed a novel method for alkaline phosphatase (ALP) assay based on the target-induced transcription of a light-up RNA aptamer, consequently producing a highly enhanced fluorescence signal upon specifically binding to the corresponding dye. Using this strategy, we successfully determined the ALP activity down to 0.018 U L-1 (dynamic linear range of 0.04-4 U L-1) with excellent selectivity.
- Publisher
- ROYAL SOC CHEMISTRY
- Issue Date
- 2021-10
- Language
- English
- Article Type
- Article
- Citation
CHEMICAL COMMUNICATIONS, v.57, no.92, pp.12341 - 12344
- ISSN
- 1359-7345
- Appears in Collection
- CBE-Journal Papers(저널논문)
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