DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Chang, Ho-Nam | - |
dc.contributor.advisor | Lee, Sang-Yup | - |
dc.contributor.advisor | 장호남 | - |
dc.contributor.advisor | 이상엽 | - |
dc.contributor.author | Lee, Pyung-Cheon | - |
dc.contributor.author | 이평천 | - |
dc.date.accessioned | 2011-12-13T01:35:19Z | - |
dc.date.available | 2011-12-13T01:35:19Z | - |
dc.date.issued | 2001 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=165764&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28861 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 화학공학과, 2001.2, [ x, 127 p. ] | - |
dc.description.abstract | Physiological and cultural characteristics of a succinate-producing Anaerobiospirillum succiniciproducens and a novel succinate-producing Mannheimia sp., isolated from bovine rumen, were studied to develop the biological systems for succinic acid production. Among several environmental and physiological factors affecting cell growth and end-product formation, the external supply of $CO_2$ and $H_2$ enhanced the growth of A. succiniciproducens and its succinic acid formation. Steady-state parameters obtained by the continuous cultures of A. succiniciproducens indicated that both succinic acid production and acetic acid production were found to be growth-associated and that the formation of unwanted acetic acid reduced the succinic acid yield and cell growth. Acetic acid formation could be avoided by using glycerol as a carbon source during the batch and continuous cultures. Accordingly, a high yield and productivity of succinic acid could be obtained. Inexpensive materials such as whey, wood hydrolysate, and corn steep liquor could be effectively utilized by A. succiniciproducens. A novel succinate-producing Mannheimia sp. 55E isolated from bovine rumen is a metabolically versatile bacterium capable of growing either anaerobically and aerobically. It fermented glucose to succinic, acetic, formic, and lactic acids in response to environmental conditions. Under 100% $CO_2$ condition, more succinic acid and less lactic acid were formed, and cells grew well. The culture pH did not affect the ratio of end-products formed under 100% $CO_2$condition. Whereas, under 100% $N_2$ condition, more lactic acid and less succinic acid were formed, and cells grew poor. Aerobically, Mannheimia sp. 55E produces lactic and acetic acids without exhausted $CO_2$ gas and irrespective of initial glucose concentration, which indicated that this bacterium may not have tricarboxylic acid cycle (TCA). The pckA gene encoding phosphoenolpyruvate carboxykinase (PEPCK), a key enzyme in the cen... | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Anaerobiospirillum succiniciproducens | - |
dc.subject | Mannheimia sp. | - |
dc.subject | 숙신산 | - |
dc.subject | Anaerobiospirillum succiniciproducens | - |
dc.subject | Succinic acid | - |
dc.subject | Mannheimia sp. | - |
dc.title | Biological production of succinic acid by anaerobiospirillum succiniciproducens and mannheimia sp. | - |
dc.title.alternative | Anaerobiospirillum succiniciproducens와 mannheimia sp.을 이용한 생물학적 숙신산 생산 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 165764/325007 | - |
dc.description.department | 한국과학기술원 : 화학공학과, | - |
dc.identifier.uid | 000975309 | - |
dc.contributor.localauthor | Chang, Ho-Nam | - |
dc.contributor.localauthor | Lee, Sang-Yup | - |
dc.contributor.localauthor | 장호남 | - |
dc.contributor.localauthor | 이상엽 | - |
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