Biological production of succinic acid by anaerobiospirillum succiniciproducens and mannheimia sp. = Anaerobiospirillum succiniciproducens와 mannheimia sp.을 이용한 생물학적 숙신산 생산

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Physiological and cultural characteristics of a succinate-producing Anaerobiospirillum succiniciproducens and a novel succinate-producing Mannheimia sp., isolated from bovine rumen, were studied to develop the biological systems for succinic acid production. Among several environmental and physiological factors affecting cell growth and end-product formation, the external supply of $CO_2$ and $H_2$ enhanced the growth of A. succiniciproducens and its succinic acid formation. Steady-state parameters obtained by the continuous cultures of A. succiniciproducens indicated that both succinic acid production and acetic acid production were found to be growth-associated and that the formation of unwanted acetic acid reduced the succinic acid yield and cell growth. Acetic acid formation could be avoided by using glycerol as a carbon source during the batch and continuous cultures. Accordingly, a high yield and productivity of succinic acid could be obtained. Inexpensive materials such as whey, wood hydrolysate, and corn steep liquor could be effectively utilized by A. succiniciproducens. A novel succinate-producing Mannheimia sp. 55E isolated from bovine rumen is a metabolically versatile bacterium capable of growing either anaerobically and aerobically. It fermented glucose to succinic, acetic, formic, and lactic acids in response to environmental conditions. Under 100% $CO_2$ condition, more succinic acid and less lactic acid were formed, and cells grew well. The culture pH did not affect the ratio of end-products formed under 100% $CO_2$condition. Whereas, under 100% $N_2$ condition, more lactic acid and less succinic acid were formed, and cells grew poor. Aerobically, Mannheimia sp. 55E produces lactic and acetic acids without exhausted $CO_2$ gas and irrespective of initial glucose concentration, which indicated that this bacterium may not have tricarboxylic acid cycle (TCA). The pckA gene encoding phosphoenolpyruvate carboxykinase (PEPCK), a key enzyme in the cen...
Chang, Ho-NamresearcherLee, Sang-Yupresearcher장호남researcher이상엽researcher
한국과학기술원 : 화학공학과,
Issue Date
165764/325007 / 000975309

학위논문(박사) - 한국과학기술원 : 화학공학과, 2001.2, [ x, 127 p. ]


Anaerobiospirillum succiniciproducens; Mannheimia sp.; 숙신산; Anaerobiospirillum succiniciproducens; Succinic acid; Mannheimia sp.

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