Tungsten trioxide (WO3) is an abundant, versatile oxide that is widely explored for catalysis, sensing, electrochromic devices, and numerous other applications. The exploitation of WO3 in nanosheet form provides potential advantages in many of these fields because the 2D structures have high surface area and preferentially exposed facets. Relative to bulk WO3, nanosheets expose more active sites for surface-sensitive sensing/catalytic reactions, and improve reaction kinetics in cases where ionic diffusion is a limiting factor (e.g. electrochromic or charge storage). Synthesis of high aspect ratio WO3 nanosheets, however, is more challenging than other 2D materials because bulk WO3 is not an intrinsically layered material, making the widely-studied sonication-based exfoliation methods used for other 2D materials not well-suited to WO3. WO3 is also highly complex in terms of how the synthesis method affects the properties of the final material. Depending on the route used and subsequent post-synthesis treatments, a wide variety of different morphologies, phases, exposed facets, and defect structures are created, all of which must be carefully considered for the chosen application. In this review, the recent developments in WO3 nanosheet synthesis and their impact on performance in various applications are summarized and critically analyzed.