DC Field | Value | Language |
---|---|---|
dc.contributor.author | Valiulahi, Parvin | ko |
dc.contributor.author | Vidyawan, Vincencius | ko |
dc.contributor.author | Puspita, Lesly | ko |
dc.contributor.author | Oh, Youjin | ko |
dc.contributor.author | Juwono, Virginia Blessy | ko |
dc.contributor.author | Sittipo, Panida | ko |
dc.contributor.author | Friedlander, Gilgi | ko |
dc.contributor.author | Yahalomi, Dayana | ko |
dc.contributor.author | Sohn, Jong Woo | ko |
dc.contributor.author | Lee, Yun Kyung | ko |
dc.contributor.author | Yoon, Jeong Kyo | ko |
dc.contributor.author | Shim, Jae-Won | ko |
dc.date.accessioned | 2021-08-30T05:10:09Z | - |
dc.date.available | 2021-08-30T05:10:09Z | - |
dc.date.created | 2021-08-26 | - |
dc.date.created | 2021-08-26 | - |
dc.date.issued | 2021-08 | - |
dc.identifier.citation | STEM CELL REPORTS, v.16, no.8, pp.1938 - 1952 | - |
dc.identifier.issn | 2213-6711 | - |
dc.identifier.uri | http://hdl.handle.net/10203/287490 | - |
dc.description.abstract | Serotonin (5-HT) neurons, the major components of the raphe nuclei, arise from ventral hindbrain progenitors. Based on anatomical location and axonal projection, 5-HT neurons are coarsely divided into rostral and caudal groups. Here, we propose a novel strategy to generate hindbrain 5-HT neurons from human pluripotent stem cells (hPSCs), which involves the formation of ventral-type neural progenitor cells and stimulation of the hindbrain 5-HT neural development. A caudalizing agent, retinoid acid, was used to direct the cells into the hindbrain cell fate. Approximately 30%-40% of hPSCs successfully developed into 5-HT-expressing neurons using our protocol, with the majority acquiring a caudal rhombomere identity (r5-8). We further modified our monolayer differentiation system to generate 5-HT neuron-enriched hindbrain-like organoids. We also suggest downstream applications of our 5-HT monolayer and organoid cultures to study neuronal response to gut microbiota. Our methodology could become a powerful tool for future studies related to 5-HT neurotransmission. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.title | Generation of caudal-type serotonin neurons and hindbrain-fate organoids from hPSCs | - |
dc.type | Article | - |
dc.identifier.wosid | 000684300500009 | - |
dc.identifier.scopusid | 2-s2.0-85112668972 | - |
dc.type.rims | ART | - |
dc.citation.volume | 16 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 1938 | - |
dc.citation.endingpage | 1952 | - |
dc.citation.publicationname | STEM CELL REPORTS | - |
dc.identifier.doi | 10.1016/j.stemcr.2021.06.006 | - |
dc.contributor.localauthor | Sohn, Jong Woo | - |
dc.contributor.nonIdAuthor | Valiulahi, Parvin | - |
dc.contributor.nonIdAuthor | Vidyawan, Vincencius | - |
dc.contributor.nonIdAuthor | Puspita, Lesly | - |
dc.contributor.nonIdAuthor | Juwono, Virginia Blessy | - |
dc.contributor.nonIdAuthor | Sittipo, Panida | - |
dc.contributor.nonIdAuthor | Friedlander, Gilgi | - |
dc.contributor.nonIdAuthor | Yahalomi, Dayana | - |
dc.contributor.nonIdAuthor | Lee, Yun Kyung | - |
dc.contributor.nonIdAuthor | Yoon, Jeong Kyo | - |
dc.contributor.nonIdAuthor | Shim, Jae-Won | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | RETINOIC ACID SYNTHESIS | - |
dc.subject.keywordPlus | BRAIN-DEVELOPMENT | - |
dc.subject.keywordPlus | HUMAN ES | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | MITOCHONDRIAL | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | DYSFUNCTION | - |
dc.subject.keywordPlus | IDENTITY | - |
dc.subject.keywordPlus | MARKER | - |
dc.subject.keywordPlus | PARKIN | - |
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