High cell density continuous culture of anchorage-dependent animal cells in a cylindrical depth filter perfusion system원통형 깊이필터를 이용한 부착성 동물세포의 고농도 연속배양

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A depth filter perfusion system (DFPS) equipped with cylindrical depth filter was applied to high cell density continuous culture of anchorage-dependent animal cells. Whole cell enzyme immobilization using a depth filter was also investigated as an application of the DFPS. Cylindrical depth filters are widely used in industries handling fluids containing solid particles. Many household water purification systems also use these cylindrical depth filters which are disposable and inexpensive, and have good filtration performances. An important feature of depth filters is that captured particles are distributed homogeneously in the filter matrix, which improves particle removal efficiency and makes long-term operation possible without filter clogging. The DFPS filter units are available commercially in large quantities at a reasonable price and they are also uniform in quality. In addition, the DFPS provides advantages of high surface-to-volume ratio of 450-600 ㎠/㎤, low cost set-up, easy operation and scale-up. To identify the basic characteristics of the DFPS, residence time distributions, surface modifications by coating various materials and oxygen transfer properties by bubble aeration were investigated. From the analysis of residence time distributions, the DFPS exhibits a plug flow behavior with some dispersion. In the 20 and 40 ㎛ filter, increase of the circulation rate did not affect significantly to the mixing patterns. However, in the 70 ㎛ filter, dispersion of the fluid decreased as increasing the circulation rate. Surface modification by gelatin coating increased the cation and anion exchange capacities of the depth filter and promoted the attachment rate of the cells tested onto the filter surfaces. Pluronic F-68 containing medium and sparging with the micron-size filter could supply enough oxygen to support a cell concentration in the order of $10^8$ cells/ml, theoretically. Vero cells and recombinant CHO cells were cultivated in the DFPS to test the ...
Chang, Ho-Namresearcher장호남researcher
한국과학기술원 : 화학공학과,
Issue Date
101912/325007 / 000895504

학위논문(박사) - 한국과학기술원 : 화학공학과, 1995.8, [ xix, 158 p. ]


Invertase; Whole Cell Enzyme Immobilization; CHO; Vero; Animal Cell Culture; Depth Filter Perfusion System; Nitrile Hydratase; 니트릴하이드라타제; 인버타제; 전세포 효소 고정화; 초 세포; 베로 세포; 동물세포배양; 깊이필터

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