DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seo, Lin | ko |
dc.contributor.author | Kim, Yong-In | ko |
dc.contributor.author | Kim, Hyoungmin | ko |
dc.contributor.author | Hyun, Kwangbeom | ko |
dc.contributor.author | Kim, Jaehoon | ko |
dc.contributor.author | Lee, J. Eugene | ko |
dc.date.accessioned | 2021-08-09T05:50:18Z | - |
dc.date.available | 2021-08-09T05:50:18Z | - |
dc.date.created | 2021-08-09 | - |
dc.date.created | 2021-08-09 | - |
dc.date.created | 2021-08-09 | - |
dc.date.created | 2021-08-09 | - |
dc.date.created | 2021-08-09 | - |
dc.date.issued | 2021-09 | - |
dc.identifier.citation | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v.1869, no.9 | - |
dc.identifier.issn | 1570-9639 | - |
dc.identifier.uri | http://hdl.handle.net/10203/287071 | - |
dc.description.abstract | Kruppel-like factor 2 (Klf2) is a DNA-binding transcription factor that regulates embryonic stem cell-specific gene expression. Transcription cofactors such as p300 acetyltransferase and Erk kinases interact with Klf2, providing an additional layer of transcription regulation in embryonic stem cells. To carry out a thorough survey of the Klf2 interactome in embryonic stem cells and identify novel transcription cofactors, we designed a modified immunoprecipitation-mass spectrometry (IP-MS) method. In this method, recombinant Klf2, expressed and purified from Sf9 insect cells instead of ectopically expressed in cells, was used as bait. Using this modified IP-MS method, we discovered nine Klf2-interacting proteins, including the previously reported Crebbp and p300. These proteins showed at least an 8-fold increase in signal intensity in Klf2 pull-downs compared with controls, with Pvalues <0.010. Among the identified Klf2-binding proteins confirmed using our IP-MS workflow was Snd1, which we found to interact directly with Klf2 and function as a transcriptional coactivator of Klf2 to drive the Oct4 gene expression. Collectively, our IP-MS protocol may offer a useful tool for identifying novel transcription cofactors in stem cells. | - |
dc.language | English | - |
dc.publisher | ELSEVIER | - |
dc.title | Discovery of Klf2 interactors in mouse embryonic stem cells by immunoprecipitation-mass spectrometry utilizing exogenously expressed bait | - |
dc.type | Article | - |
dc.identifier.wosid | 000668998900001 | - |
dc.identifier.scopusid | 2-s2.0-85106608511 | - |
dc.type.rims | ART | - |
dc.citation.volume | 1869 | - |
dc.citation.issue | 9 | - |
dc.citation.publicationname | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | - |
dc.identifier.doi | 10.1016/j.bbapap.2021.140672 | - |
dc.contributor.localauthor | Kim, Jaehoon | - |
dc.contributor.nonIdAuthor | Kim, Yong-In | - |
dc.contributor.nonIdAuthor | Lee, J. Eugene | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Mouse embryonic stem cells | - |
dc.subject.keywordAuthor | Klf2 | - |
dc.subject.keywordAuthor | Immunoprecipitation-mass spectrometry | - |
dc.subject.keywordAuthor | Snd1 | - |
dc.subject.keywordAuthor | Exogenous bait | - |
dc.subject.keywordPlus | COMPUTATIONAL PLATFORM | - |
dc.subject.keywordPlus | GROUND-STATE | - |
dc.subject.keywordPlus | RNA-BINDING | - |
dc.subject.keywordPlus | SND1 ACTS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | ACETYLATION | - |
dc.subject.keywordPlus | TGF-BETA-1 | - |
dc.subject.keywordPlus | EKLF | - |
dc.subject.keywordPlus | EBP1 | - |
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