All-in-One DNA Extraction Tube for Facilitated Real-Time Detection of Infectious Pathogens

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y An "all-in-one tube" platform is developed, where the genetic analysis involving DNA extraction, amplification, and detection can be performed in a single tube. The all-in-one tube consists of a polymerase chain reaction (PCR) tube in which the inner surface is conformally modified with a tertiary-amine-containing polymer to generate a strong electrostatic interaction with DNA. The all-in-one tube provides high DNA capture efficiency exceeding 80% from Escherichia coli O157: H7 pathogen at a wide range of DNA amount from 0.003 to 3 ng. Indeed, the use of the surface-functionalized PCR tube enables direct amplification and detection of the surface-captured DNA without the modification of standard real-time PCR instrument. Besides, this platform has sensitivity, selectivity, and reliability enough for accurate detection at the minimal infective dose of both gram-positive and negative pathogens. The all-in-one tube enables the direct molecular diagnosis, substantially reducing the labor-intensive pathogen detection steps while providing high compatibility with the currently established real-time PCR instruments, and illustrates its on-site applicability with convenience expandable to various genetic analyses including food safety testing, forensic analysis, and clinical diagnosis.
Publisher
WILEY
Issue Date
2021-07
Language
English
Article Type
Article
Citation

ADVANCED HEALTHCARE MATERIALS, v.10, no.14, pp.2100430

ISSN
2192-2640
DOI
10.1002/adhm.202100430
URI
http://hdl.handle.net/10203/286931
Appears in Collection
CBE-Journal Papers(저널논문)
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