DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Sungyul | ko |
dc.contributor.author | Lee, Young-suk | ko |
dc.contributor.author | Choi, Yeon | ko |
dc.contributor.author | Son, Ahyeon | ko |
dc.contributor.author | Park, Youngran | ko |
dc.contributor.author | Lee, Kyung-Min | ko |
dc.contributor.author | Kim, Jeesoo | ko |
dc.contributor.author | Kim, Jong-Seo | ko |
dc.contributor.author | Kim, V. Narry | ko |
dc.date.accessioned | 2021-07-29T03:10:09Z | - |
dc.date.available | 2021-07-29T03:10:09Z | - |
dc.date.created | 2021-07-29 | - |
dc.date.created | 2021-07-29 | - |
dc.date.issued | 2021-04 | - |
dc.identifier.citation | MOLECULAR CELL, v.81, no.13, pp.2838 - + | - |
dc.identifier.issn | 1097-2765 | - |
dc.identifier.uri | http://hdl.handle.net/10203/286898 | - |
dc.description.abstract | SARS-CoV-2 is an RNA virus whose success as a pathogen relies on its abilities to repurpose host RNA -binding proteins (RBPs) and to evade antiviral RBPs. To uncover the SARS-CoV-2 RNA interactome, we here develop a robust ribonucleoprotein (RNP) capture protocol and identify 109 host factors that directly bind to SARS-CoV-2 RNAs. Applying RNP capture on another coronavirus, HCoV-OC43, revealed evolutionarily conserved interactions between coronaviral RNAs and host proteins. Transcriptome analyses and knockdown experiments delineated 17 antiviral RBPs, including ZC3HAV1, TRIM25, PARP12, and SHFL, and 8 pro viral RBPs, such as EIF3D and CSDE1, which are responsible for co-opting multiple steps of the mRNA life cycle. This also led to the identification of LARP1, a downstream target of the mTOR signaling pathway, as an antiviral host factor that interacts with the SARS-CoV-2 RNAs. Overall, this study provides a comprehensive list of RBPs regulating coronaviral replication and opens new avenues for therapeutic interventions. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.title | The SARS-CoV-2 RNA interactome | - |
dc.type | Article | - |
dc.identifier.wosid | 000671130000003 | - |
dc.identifier.scopusid | 2-s2.0-85105795237 | - |
dc.type.rims | ART | - |
dc.citation.volume | 81 | - |
dc.citation.issue | 13 | - |
dc.citation.beginningpage | 2838 | - |
dc.citation.endingpage | + | - |
dc.citation.publicationname | MOLECULAR CELL | - |
dc.identifier.doi | 10.1016/j.molcel.2021.04.022 | - |
dc.contributor.localauthor | Lee, Young-suk | - |
dc.contributor.nonIdAuthor | Lee, Sungyul | - |
dc.contributor.nonIdAuthor | Choi, Yeon | - |
dc.contributor.nonIdAuthor | Son, Ahyeon | - |
dc.contributor.nonIdAuthor | Park, Youngran | - |
dc.contributor.nonIdAuthor | Lee, Kyung-Min | - |
dc.contributor.nonIdAuthor | Kim, Jeesoo | - |
dc.contributor.nonIdAuthor | Kim, Jong-Seo | - |
dc.contributor.nonIdAuthor | Kim, V. Narry | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | CORONAVIRUS | - |
dc.subject.keywordPlus | TRANSLATION | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | INTERFERON | - |
dc.subject.keywordPlus | NSP9 | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | REPLICATION | - |
dc.subject.keywordPlus | MODULATION | - |
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