Surface modifications and stabilities of liposomal systems as carriers of drug and vaccine = 약물과 백신의 운반체로서의 리포솜의 표면 수식과 안정성에 관한 연구

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The surface modification and the stabilities of antibody bound liposomes were investigated. Unsaturated PE, which does not formed stable bilayer at the physiological condition, was made stable bilayer through introducing $2.4 \times 10^{-4}$ molar ratio of the palmitoyl immunoglobulin G(p-IgG) into the lipid bilayer, and the formation of immunoliposomes was confirmed by the papain-induced lysis of calcein-entrapped immunoliposomes. The turbidity change and the release of calcein from liposomes were measured with the bile salts added to investigate the solubilization of liposomes. The solubilization of liposomes by bile salts depends upon the number of hydroxyl groups in the cholate. The solubilizing phenomena were significantly influenced by conjugated bile salts, and the number of hydroxyl groups in bile salts, but not by the configurational states of the hydroxyl group. Increase in hydrophobicity of bile salts resulted in the decrease of half-maximal concentrations for both absorbance and calcein release. The substitution of the head group with glycine or taurine decreased the calcein release, but the absorbance showed the different behavior. The stabilities of immunoliposomes incorporated with variable amounts of ganglioside $G_{M1$, were investigated as a function of time and temperature. DOPE immunoliposome was destabilized within several hours, but the stabilities of liposomes were enhanced by the incorporation of ganglioside $G_{M1}$ into immunoliposome. sensitive to the Fab`` coupled to the vesicle. As time elapsed, the turbidities of Fab`` vesicle and MPB-PE vesicle did not change, but the turbidity of liposomes manufactured by sonication method had changed significantly. Such phenomena may be attributed to the disturbance of the bilayer characteristics. The complex and physical characteristics of egg phosphatidylcholine(PC) liposome containing amphotericin B were investigated in terms of the size distribution, the calcein release, the turbidity, and t...
Kim, Jong-Dukresearcher김종득researcher
한국과학기술원 : 화학공학과,
Issue Date
69705/325007 / 000885359

학위논문(박사) - 한국과학기술원 : 화학공학과, 1994.8, [ xxii, 202 p. ]

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