Robust Biocatalysts Displayed on Crystalline Protein-Layered Cells for Efficient and Sustainable Hydration of Carbon Dioxide

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dc.contributor.authorKoo, Bon Ilko
dc.contributor.authorChoi, Jae Woongko
dc.contributor.authorSong, Seuk Youngko
dc.contributor.authorChoi, Ye Hunko
dc.contributor.authorLee, Tae Yongko
dc.contributor.authorKim, Shin-Hyunko
dc.contributor.authorJeong, Ki Junko
dc.contributor.authorNam, Yoon Sungko
dc.date.accessioned2021-06-23T02:30:19Z-
dc.date.available2021-06-23T02:30:19Z-
dc.date.created2021-04-26-
dc.date.issued2021-06-
dc.identifier.citationADVANCED FUNCTIONAL MATERIALS, v.31, no.25, pp.2102497-
dc.identifier.issn1616-301X-
dc.identifier.urihttp://hdl.handle.net/10203/286110-
dc.description.abstractMineral carbonation is the most effective carbon capture technique, but carbon dioxide (CO2) conversion is limited by the slow hydration rate of CO2 (<10(-1) s(-1)). A biological solution exists: carbonic anhydrase (CA) efficiently hydrates CO2 at a turnover rate of approximate to 10(6) s(-1) under ambient conditions, making it an extremely attractive candidate for industrial post-combustion CO2 capture. However, high cost and poor long-term stability impose a technical barrier to its practical uses. Here, a genetically engineered Corynebacterium glutamicum (C. glutamicum) is introduced as a robust cell display platform for the in situ stabilization and low-cost production of CA. The enzyme is displayed in the mycolic layer with porin B as an anchoring protein with (GGGGS)(2) as a spacer. The cell-displayed CA exhibits no significant inactivation of the CO2 hydration activity for at least one month at 37 degrees C. Its denaturation rate constant at 50 degrees C (0.07) is an order of magnitude lower than that of free CA (0.52-0.54). This study demonstrates that a structurally robust cell template allows the effective stabilization of CA, suggesting the C. glutamicum-based cell display as a promising technique to achieve highly efficient, sustainable, and low-cost CO2 capture for industrial applications.-
dc.languageEnglish-
dc.publisherWILEY-V C H VERLAG GMBH-
dc.titleRobust Biocatalysts Displayed on Crystalline Protein-Layered Cells for Efficient and Sustainable Hydration of Carbon Dioxide-
dc.typeArticle-
dc.identifier.wosid000640566600001-
dc.identifier.scopusid2-s2.0-85104312009-
dc.type.rimsART-
dc.citation.volume31-
dc.citation.issue25-
dc.citation.beginningpage2102497-
dc.citation.publicationnameADVANCED FUNCTIONAL MATERIALS-
dc.identifier.doi10.1002/adfm.202102497-
dc.contributor.localauthorKim, Shin-Hyun-
dc.contributor.localauthorJeong, Ki Jun-
dc.contributor.localauthorNam, Yoon Sung-
dc.contributor.nonIdAuthorSong, Seuk Young-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorcarbon dioxide capture-
dc.subject.keywordAuthorcarbonic anhydrase-
dc.subject.keywordAuthorcell display-
dc.subject.keywordAuthorCorynebacterium-
dc.subject.keywordAuthorenzyme thermostability-
Appears in Collection
CBE-Journal Papers(저널논문)MS-Journal Papers(저널논문)
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