Robust Biocatalysts Displayed on Crystalline Protein-Layered Cells for Efficient and Sustainable Hydration of Carbon Dioxide

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Mineral carbonation is the most effective carbon capture technique, but carbon dioxide (CO2) conversion is limited by the slow hydration rate of CO2 (<10(-1) s(-1)). A biological solution exists: carbonic anhydrase (CA) efficiently hydrates CO2 at a turnover rate of approximate to 10(6) s(-1) under ambient conditions, making it an extremely attractive candidate for industrial post-combustion CO2 capture. However, high cost and poor long-term stability impose a technical barrier to its practical uses. Here, a genetically engineered Corynebacterium glutamicum (C. glutamicum) is introduced as a robust cell display platform for the in situ stabilization and low-cost production of CA. The enzyme is displayed in the mycolic layer with porin B as an anchoring protein with (GGGGS)(2) as a spacer. The cell-displayed CA exhibits no significant inactivation of the CO2 hydration activity for at least one month at 37 degrees C. Its denaturation rate constant at 50 degrees C (0.07) is an order of magnitude lower than that of free CA (0.52-0.54). This study demonstrates that a structurally robust cell template allows the effective stabilization of CA, suggesting the C. glutamicum-based cell display as a promising technique to achieve highly efficient, sustainable, and low-cost CO2 capture for industrial applications.
Publisher
WILEY-V C H VERLAG GMBH
Issue Date
2021-06
Language
English
Article Type
Article
Citation

ADVANCED FUNCTIONAL MATERIALS, v.31, no.25, pp.2102497

ISSN
1616-301X
DOI
10.1002/adfm.202102497
URI
http://hdl.handle.net/10203/286110
Appears in Collection
CBE-Journal Papers(저널논문)MS-Journal Papers(저널논문)
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