DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Mi Gyeom | ko |
dc.contributor.author | Kim, Che Lin | ko |
dc.contributor.author | Kim, Young Sik | ko |
dc.contributor.author | Jang, Ju Woong | ko |
dc.contributor.author | Lee, Gyun Min | ko |
dc.date.accessioned | 2021-06-15T01:50:12Z | - |
dc.date.available | 2021-06-15T01:50:12Z | - |
dc.date.created | 2021-06-08 | - |
dc.date.created | 2021-06-08 | - |
dc.date.created | 2021-06-08 | - |
dc.date.issued | 2021-02 | - |
dc.identifier.citation | SCIENTIFIC REPORTS, v.11, no.1 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/10203/285902 | - |
dc.description.abstract | Cell surface heparan sulfate proteoglycan (HSPG)-mediated endocytosis results in poor yields of recombinant human bone morphogenetic proteins (rhBMPs) from CHO cell cultures. Upon incubation of rhBMP-2 and rhBMP-7 with CHO cells at 37 °C, both rhBMP-2 and rhBMP-7 bound to the cell surface HSPGs in CHO cells, but only rhBMP-2 was actively internalized into CHO cells. Cell surface HSPGs were found to serve as the main receptor for rhBMP-2 internalization. It was also found that the cell surface HSPG-mediated endocytosis of rhBMP-2 occurred through both the clathrin- and caveolin-dependent pathways. Blockage of rhBMP-2 internalization by the addition of structural analogs of HSPGs such as dextran sulfate (DS) and heparin dramatically increased rhBMP-2 production in recombinant CHO (rCHO) cell cultures. Compared to the control cultures, addition of DS (1.0 g/L) and heparin (0.2 g/L) resulted in a 22.0- and 19.0-fold increase in the maximum rhBMP-2 concentration, respectively. In contrast, the production of rhBMP-7, which was not internalized into the rCHO cells, did not dramatically increase upon addition of DS and heparin. Taken together, rhBMPs have a different fate in terms of HSPG-mediated internalization in CHO cells. HSPG-mediated endocytosis of each rhBMP should be understood individually to increase the rhBMP yield in rCHO cell cultures. | - |
dc.language | English | - |
dc.publisher | NATURE RESEARCH | - |
dc.title | Selective endocytosis of recombinant human BMPs through cell surface heparan sulfate proteoglycans in CHO cells: BMP-2 and BMP-7 | - |
dc.type | Article | - |
dc.identifier.wosid | 000684869100003 | - |
dc.identifier.scopusid | 2-s2.0-85100719203 | - |
dc.type.rims | ART | - |
dc.citation.volume | 11 | - |
dc.citation.issue | 1 | - |
dc.citation.publicationname | SCIENTIFIC REPORTS | - |
dc.identifier.doi | 10.1038/s41598-021-82955-1 | - |
dc.contributor.localauthor | Lee, Gyun Min | - |
dc.contributor.nonIdAuthor | Kim, Che Lin | - |
dc.contributor.nonIdAuthor | Kim, Young Sik | - |
dc.contributor.nonIdAuthor | Jang, Ju Woong | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | BONE MORPHOGENETIC PROTEINS | - |
dc.subject.keywordPlus | FIBROBLAST-GROWTH-FACTOR | - |
dc.subject.keywordPlus | MEDIATED ENDOCYTOSIS | - |
dc.subject.keywordPlus | INTERNALIZATION | - |
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