Effects of different mRNA 3'-untranslated regions on hammerhead ribozyme efficiency in vivo = 전령 RNA의 3'-비번역 염기열에 따른 생체내 망치머리형 리보핵산효소 효율의 변화

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 358
  • Download : 0
880-01The efficiency of ribozyme function depends on the promoter as well as the stability and localization of the transcript. Thus expression cassette determine the functional activity of ribozymes by controlling their intracellular colocalization. We have studied hammerhead ribozyme function suppressing Hepatitis B Virus X gene. Especially, we have designed the expression cassette including β- and γ-actin 3-UTR(untranslated region). Actin 3-UTR sequences are responsible for localization of mRNA and we acquired these sequences by PCR with HeLa cDNA library. pRc/RSV was used as mammalian cell expression vector and several plasmids containing riobzyme and target flanked by each 3-UTR were constructed. After each kind of ribozyme and target were paired off, these vectors were transiently transfected into HeLa cell and ribozyme activities were detected by β-galactosidase assay. When the cells were transfected with vectors including 3-UTR in either target or ribozyme, the activity of ribozyme was reduced. But the cells were tranfected with vectors including the same kind of 3-UTR in both target and ribozyme, the activity of ribozyme was slightly enhanced.
Advisors
Chung, Jong-KyeongresearcherKang, Chang-Wonresearcher정종경researcher강창원researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
1999
Identifier
151574/325007 / 000973344
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 1999.2, [ [v], 48 p. ]

Keywords

3``-untranslated region; Localization; 망치머리형 리보핵산 효소; 액틴; 3``-비번역 부위; 배치; Hammerhead ribozyme; Actin

URI
http://hdl.handle.net/10203/28582
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=151574&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0