cDNA cloning of guamerin and nucleotide sequence거머린의 cDNA 클로닝과 염기서열

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A cDNA library was constructed from the Korean native leeches, Hirudo nipponia using the PCR-directed method. A full-length cDNA encoding guamerin was isolated from the cDNA library and nucleotide sequence was analyzed. The cDNA contained an open reading frame of 228 bp which encoded a 75 amino acid protein. The guamerin precursor consisted of additional 5 amino acid residues at N-terminal region and 13 amino acid residues at C-terminal region. The mature guamerin consisted of 57 amino acids. The deduced amino acid sequence revealed a different amino acid residue from that of guamerin. This cDNA has 71 % local identity to cDNA of antistasin, factor Xa inhibitor derived leech, Haementaria officialis at the DNA level.
Advisors
Kang, Ke-Won강계원
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
1998
Identifier
135372/325007 / 000963614
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 1998.2, [ iv, 30 p. ]

Keywords

Guamerin; cDNA; Library; 염기서열; cDNA 라이브러리; 거머린; Nucleotide sequence

URI
http://hdl.handle.net/10203/28565
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=135372&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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