Genetic evidence of a novel ribose transport system in escherichia coli K-12 = 대장균에 존재하는 새로운 리보스 수송계의 발견

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 290
  • Download : 0
Ribose is transported through three known sugar transporters; the rbs operon (high-affinity D-ribose transporter), the als operon(D-allose transporter) and the xyl operon(D-xylose transporter). Mutations enhancing growth arise spontaneously at the frequencies of $10^{-8} - 10^{-9}$ in ribose minimal plate from the strain lacking the rbs transport system. Genetic analysis revealed that their growth phenotypes were not dependent on the three known mechanisms and that rbsD and rbsK, the components of rbs operon, were essential. Growth of the mutants on D-ribose medium showed that their growth rates were about 80% of that of wild type with the intact rbs system. Although ribose uptake rate of the mutant was much lower than that of binding protein dependent transporter, the mutant showed better uptake rate than those of als operon and xyl operon. Genetic tagging and mapping analyses revealed that two loci from the E.coli linkage map are involved in the novel ribose transport mechanism. One was found at 25 min and the other was at 36min of E.coli strain OW1.
Advisors
Park, Chan-KyuresearcherChung, Jong-Kyeongresearcher박찬규researcher정종경researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
1998
Identifier
135367/325007 / 000963382
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 1998.2, [ iv, 35 p. ]

Keywords

rbsD; rbs operon; 리보스 수송; 대장균; rbsD; rbs 오페론; Ribose transport; Escherichia coli

URI
http://hdl.handle.net/10203/28560
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=135367&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0