A water-soluble boronate masked benzoindocyanin fluorescent probe for the detection of endogenous mitochondrial peroxynitrite in live cells and zebrafish as inflammation models

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Endogenous peroxynitrite a highly reactive oxidant plays significant roles in various physiopathological processes in living organisms. Therefore, sensing and monitoring of peroxynitrite with accurate detection specifically, is an immense challenge. To address this issue, we synthesized a water soluble, mitochondria-targeting "turn-on" fluorescent probe (BICBzBF) for the detection of mitochondrial peroxynitrite. BICBzBF (pale yellow) demonstrated high sensitivity and selectivity in fluorometric tracing of peroxynitrite over other ROS/RNS tested. This arylboronate-masked probe responds to peroxynitrite, resulting in the corresponding phenol derivative; a strong fluorescence accompanied a change to red rose as observed by the naked eye. The probe responds to peroxynitrite within <120 s, shows a lower nanomolar detection limit (60.5 nM), exhibits a remarkable ca. 32fold intense increase. Taking these features fully into account, BICBzBF was evaluated in how it monitors peroxynitrite exogenously and endogenously. Moreover, BICBzBF successfully traced ONOO- in inflammatory living zebrafish animal model which are chemically induced for inflammation. The results obtained in both exogenous and endogenous studies demonstrate that BICBzBF actively detects ONOO-; major accumulation in retina and intestine indicate BICBzBF could serve as a potential molecular imaging tool in investigations of the role(s) played by peroxynitrite in a variety of physiological and pathological contexts.
Publisher
ELSEVIER SCI LTD
Issue Date
2021-07
Language
English
Article Type
Article
Citation

DYES AND PIGMENTS, v.191, pp.109371

ISSN
0143-7208
DOI
10.1016/j.dyepig.2021.109371
URI
http://hdl.handle.net/10203/285566
Appears in Collection
CBE-Journal Papers(저널논문)CH-Journal Papers(저널논문)
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