(A) study on the disulfide bonds of guamerin, a protease inhibitor from a Korean native leech = 한국거머리에서 분리한 단백질분해효소 억제제, 거머린의 이황화 결합에 관한 연구

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 586
  • Download : 0
Guamerin, a humam leukocyte elastase inhibitor from the Korean leech Hirudo nipponia is composed of 57 amino acid residues with ten cysteines. Disulfide bonds between two cystein residues were analyzed by biochemical methods. Thermolysin digestion of guamerin revealed that it had two domains containing two and three disulfide bonds, respectively. The N-terminal domain was revealed to have Cys1-Cys3 bond(disulfide bond between the first cysteine and the third cysteine) and Cys2-Cys4 bond. The disulfide bond pattern of the C-terminal domain was one of ``Cys5-Cys8, Cys6-Cys9 and Cys7-Cys10`` or ``Cys5-Cys8, Cys6-Cys10 and Cys7-Cys9`` patterns, that was not completely determined yet. Among the five disulfide bonds, there were two weak disulfide bonds more easily modified by 4-vinylpyridine at pH8.0. Likewise, recombinant guamerin expressed in the yeast Pichia pastoris and guamerin II, a serine protease inhibitor from another Korean leech, Whitmania edentula were revealed to have identical disulfide bond pattern and characteristics with guamerin. Furthermore, their disulfide bond patterns were consistent with those of hirustasin and antistasin, another serine protease inhibitors from other leech species, of which three dimensional structures were already determined by X-ray crystallography.
Advisors
Kang, Ke-Won강계원
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
1998
Identifier
135349/325007 / 000963004
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 1998.2, [ v, 37 p. ]

Keywords

Protease inhibitor; Cysteine; Disulfide; Guamerin; Leech; 거머리; 단백질분해효소 억제제; 시스테인; 이황화결합; 거머린

URI
http://hdl.handle.net/10203/28542
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=135349&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0