Identification of novel genes induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin using differential display method = Differential display 방법을 통한 2,3,7,8-tetrachlorodibenzo-p-dioxin에 의해 유도되는 유전자의 확인

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TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin; dioxin) and related halogenated aromatic hydrocarbons elicit a diverse spectrum of biochemical and toxic responses in laboratory animals and mammalian cells in culture. But the molecular mechanism of TCDD toxicity is still poorly understood. Here, we found that the genes differentially expressed by TCDD. Treatment of HepG2 cells with TCDD followed by differential display analysis showed a significantly different mRNA expression pattern from the untreated cells. The differentially displayed bands were isolated and used as probes in dot blot and northern blot analysis. Only two among 35 isolated differentially displayed bands were positive in dot blot and northern blot analysis. The DNA sequences of two clones were analyzed and compared to previously reported sequences in Genebank through computer search. This Genebank search analysis revealed that one clone is highly homologous with RanBP2 (Ras-related nuclear protein binding protein2; 93%) and the other is an unknown gene. It has been known that RanBP2 has a critical function in signal pathway through nuclear transport. Thus, it is supposed that the identified gene in this study may be a clue elucidating TCDD toxic mechanism.
Advisors
Yang, Kyu-Hwan양규환
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
1998
Identifier
135347/325007 / 000953053
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 1998.2, [ v, 35 p. ]

Keywords

Differential display analysis; TCDD(2,3,7,8-tetrachlorodibenzo-p-dioxin); RanBP2(Ras-related nuclear protein binding protein2); 랜비피투; 디퍼런셜디스플레이방법; 다이옥신

URI
http://hdl.handle.net/10203/28540
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=135347&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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