Inhibitory effect of hemin on the mutagenicity of electrophilic sulfuric acid ester of 6-hydroxymethylbenzo[a]pyrene6-Hydroxymethylbenzo[a]pyrene의 친전자성 Sulfuric Acid Ester가 일으키는 돌연변이에 대한 헤민의 억제효과

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Porphyrin compounds have been known to inhibit mutations induced by several chemical carcinogens. The chemopreventive activity of porphyrin compounds were monitored by using 6-sulfooxymethylbenzo[a]pyrene (SMBP), which is an ultimate metabolite of benzo[a]pyrene. The porphyrin compounds were quite effective in reducing both cytotoxicity and mutagenicity for SMBP at the concentration of 10㎛. Their protective effect against the mutagenicity of SMBP was in the following decreasing order: hemin, chlorophyllin, biliverdin, protoporphyrin, chlorophyll b and chlorophyll a. The inhibitory patterns of hemin for SMBP was also confirmed in S. typhimurium strains TA98 and 100. Mutation frequency caused by SMBP was diminished almost to control level at 5 nmol hemin concentration. The inhibitory effects of hemin against the assault of SMBP in V79 cells was found to related to the reduced cellular uptake of SMBP and further the remarkably lowered DNA adducts. Furthermore, hemin suppresed the hepatic cytosol and PAPS-mediated covalent binding of HMBP to DNA, possibly through inhibition of hydroxysteroid sulfotransferase, which is responsible for the formation of sulfuric acid ester from the parent alcohol. The complex formation of hemin with SMBP was confirmed by solvent extraction and the hydrolysis product identified by reverse-phase HPLC. Such a complex formation will lead to decreased cellular uptake of SMBP. The inhibition of enzyme and the inactivation of sulfur acid ester metabolite are possible chemopreventive mechanisms of porphyrin compound against SMBP.
Advisors
Chung, An-Sik정안식
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
1997
Identifier
112758/325007 / 000953660
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 1997.2, [ vii, 55 p. ]

Keywords

6-Sulfooxymethylbenzo[a]pyrene; Mutagenicity; Hemin; Hydroxysteroid sulfotransferase; 하이드록시 설포트렌스퍼레이즈; 6-설폭시메칠벤조파이렌; 돌연변이; 헤민

URI
http://hdl.handle.net/10203/28535
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=112758&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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