DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hong, Cheol Am | ko |
dc.contributor.author | Park, Jae Chul | ko |
dc.contributor.author | Na, Hyebin | ko |
dc.contributor.author | Jeon, Huiju | ko |
dc.contributor.author | Nam, Yoon Sung | ko |
dc.date.accessioned | 2021-05-25T05:10:24Z | - |
dc.date.available | 2021-05-25T05:10:24Z | - |
dc.date.created | 2021-05-25 | - |
dc.date.created | 2021-05-25 | - |
dc.date.issued | 2021-06 | - |
dc.identifier.citation | BIOSENSORS & BIOELECTRONICS, v.182 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/10203/285316 | - |
dc.description.abstract | Fast, sensitive, specific, and user-friendly DNA assay is a key technique for the next generation point-of-care molecular diagnosis. However, high-cost, time-consuming, and complicated enzyme-based DNA amplification step is essential to achieve high sensitivity. Herein, a short target DNA-catalyzed formation of quantum dot (QD)DNA hydrogel is proposed as a new DNA assay platform satisfying the above requirements. A single-stranded target DNA catalyzes the opening cycle of DNA hairpin loops, which are quickly self-assembled with DNAfunctionalized QDs to generate QD-DNA hydrogel. The three-dimensional hydrogel network allows efficient resonance energy transfer, dramatically lowering the limit of detection down to similar to 6 fM without enzymatic DNA amplification. The QD-DNA hydrogel also enables a rapid detection (1 h) with high specificity even for a singlebase mismatch. The clinical applicability of the QD-DNA hydrogel is demonstrated for the Klebsiella pneumoniae carbapenemase gene, one of the key targets of drug-resistant pathogenic bacteria. | - |
dc.language | English | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Short DNA-catalyzed formation of quantum dot-DNA hydrogel for enzyme-free femtomolar specific DNA assay | - |
dc.type | Article | - |
dc.identifier.wosid | 000642450900006 | - |
dc.identifier.scopusid | 2-s2.0-85103314691 | - |
dc.type.rims | ART | - |
dc.citation.volume | 182 | - |
dc.citation.publicationname | BIOSENSORS & BIOELECTRONICS | - |
dc.identifier.doi | 10.1016/j.bios.2021.113110 | - |
dc.contributor.localauthor | Nam, Yoon Sung | - |
dc.contributor.nonIdAuthor | Hong, Cheol Am | - |
dc.contributor.nonIdAuthor | Jeon, Huiju | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Quantum dot-DNA hydrogel | - |
dc.subject.keywordAuthor | DNA assay | - |
dc.subject.keywordAuthor | Forster resonance energy transfer | - |
dc.subject.keywordAuthor | Toehold-mediated strand displacement | - |
dc.subject.keywordPlus | ROLLING CIRCLE AMPLIFICATION | - |
dc.subject.keywordPlus | HYDROLYZING BETA-LACTAMASE | - |
dc.subject.keywordPlus | CELL-FREE DNA | - |
dc.subject.keywordPlus | ISOTHERMAL AMPLIFICATION | - |
dc.subject.keywordPlus | DIAGNOSTIC ASSAY | - |
dc.subject.keywordPlus | MICRORNA | - |
dc.subject.keywordPlus | INTEGRATION | - |
dc.subject.keywordPlus | RESOLUTION | - |
dc.subject.keywordPlus | DETECT | - |
dc.subject.keywordPlus | SERUM | - |
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