3d refractive index tomography and structured illumination microscopy system using wavefront shaper and method thereof

Abstract

An ultra-high-speed three-dimensional refractive index image shooting and fluorescent structured light illuminating microscope system using a wave surface shaper and a using method thereof are disclosed. The use method of the ultra-high-speed three-dimensional refractive index image shooting and fluorescent structured light illuminating microscope system by using the wave surface shaper comprises the following steps of: adjusting the irradiation angle of the plane wave incident to the sample by using a wave surface shaper; determining a two-dimensional light field through the sample based on an illumination angle of the plane wave; and acquiring a three-dimensional refractive index image by utilizing an optical diffraction tomography method or a filtering back projection algorithm based on the measured two-dimensional light field information.