Two forms of rat phospholipase C-b3 generated by alternative splicing = 쥐 포스포리파아제 C-b3 mRNA의 두가지 유형

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Phospholipase C-β3 is a crucial signal transduction enzyme that catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate($PIP_2$) to produce two second messenger molecules, inositol 1,4,5-triphosphate($IP_3$) and diacylglycerol(DAG). During we performed cDNA cloning studies of PLC-β3 in rat brain by screening, A full length cDNA clone(4176 base) and a shorter cDNA clone(3955 base), which lacked the region(201 bases) corresponding to PH domain at the N-terminal were isolated. Using RT-PCR, we confirmed that alternatively splicing mRNAs were generated by co- transcription from the same gene in various tissues. Relative amount of these mRNAs was determined by RNase protection assay and the ratio of the major mRNA to the minor mRNA was estimated to be 1:2.5, 1:1, 1:5, 1:4.5, 1:1.7, 1:2.5 in brain, heart, kidney, liver, lung, and testis, respectively. Also, polyclonal antibodies were raised against synthetic peptides including the PH domain portions of PLC-β3. Western blot analysis with these antibodies revealed that 150kD protein was only expressed in tissues. Supposed that two mRNA were translated in tissues, we postulate that the protein which lacks PH domain has another unknown function in the cytosol, because PH domain is essential to the role of PLC-β3.
Advisors
Yoo, Ook-Joonresearcher유욱준researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
1997
Identifier
112660/325007 / 000953355
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 1997.2, [ viii, 57 p. ]

Keywords

mRNA; Splicing; Phospholipase; Rat; 쥐; 전령알엔에이; 스플라이싱; 포스포리파아제

URI
http://hdl.handle.net/10203/28523
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=112660&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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