DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Yang, Kyu-Hwan | - |
dc.contributor.advisor | 양규환 | - |
dc.contributor.author | Suh, Jae-Hong | - |
dc.contributor.author | 서재홍 | - |
dc.date.accessioned | 2011-12-12T09:01:13Z | - |
dc.date.available | 2011-12-12T09:01:13Z | - |
dc.date.issued | 1997 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=112656&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28519 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물과학과, 1997.2, [ vii, 53 p. ] | - |
dc.description.abstract | IL-2 plays a central role in controlling the magnitude of immune response by interacting with its specific receptor (IL-2R) on cell surface. As the expressions of IL-2 and IL-2Rα, one of the three subunits of IL-2R, are controlled almost entirely at transcriptional level, any stimulus inhibiting IL-2 or IL-2Rα mRNA expression exerts profound effects on host immune system. In this study, the effects of various kinds of immunosuppressive agents on IL-2 and IL-2Rα mRNA expression were analyzed in mouse splenocytes using competitive RT-PCR method. The accuracy of this PCR-based analysis method, developed in this study, was verified by the linearity between the amounts of input template and that of the resulting PCR product. After activation with Con A (2㎍/ml), IL-2 mRNA levels peaked at 8 hr, while IL-2Rα mRNA transcripts increased gradually until 80 hr. Cyclosporin A, a well-known immunosuppressive drug, markedly inhibited IL-2Rα expression, as well as IL-2, at very low concentration (10 ng/ml). AAF and IQ also reduced the mRNA expression of both, IL-2 and IL-2Rα, to less than 30% of induced level. In the analysis of TCDD, no significant decrease in IL-2 mRNA transcript was detected. Among other chemicals (aflatoxin $B_1$, cyclophosphamide, DMBA) which need metabolic activation to exert their immunosuppressive effects, only DMBA showed inhibitory effects on IL-2 mRNA transcription after coculture with mouse hepatocytes. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Interleukin-2 receptor $\alpha$ | - |
dc.subject | Interleukin-2 | - |
dc.subject | Immunosuppressive agents | - |
dc.subject | Competitive RT-PCR | - |
dc.subject | mRNA | - |
dc.subject | mRNA | - |
dc.subject | Interleukin-2 receptor $\alpha$ | - |
dc.subject | Interleukin-2 | - |
dc.subject | 면역억제제 | - |
dc.subject | Competitive RT-PCR | - |
dc.title | Effects of immunosuppressive agents on interleukin-2 and interleukin-2 receptor α mRNA expression | - |
dc.title.alternative | 면역억제제들이 Interleukin-2 와 Interleukin-2 Receptor α의 mRNA 발현에 미치는 영향 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 112656/325007 | - |
dc.description.department | 한국과학기술원 : 생물과학과, | - |
dc.identifier.uid | 000953272 | - |
dc.contributor.localauthor | Yang, Kyu-Hwan | - |
dc.contributor.localauthor | 양규환 | - |
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