DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kim, Hyoung-Man | - |
dc.contributor.advisor | 김형만 | - |
dc.contributor.author | Kim, Joon-Sik | - |
dc.contributor.author | 김준식 | - |
dc.date.accessioned | 2011-12-12T09:01:09Z | - |
dc.date.available | 2011-12-12T09:01:09Z | - |
dc.date.issued | 1997 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=112651&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28514 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물과학과, 1997.2, [ vi, 48 p. ] | - |
dc.description.abstract | A mature mutant ribose binding protein (RBP) of Escherichia coli was obtained by site-directed mutagenesis, replacing Thr-3 in the N-domain of wild-type mature RBP (WT-mRBP) with a Trp residue (N-Trp-mRBP). The equilibrium unfolding properties and the refolding kinetics of this protein were monitored by fluorescence and circular dichroism (CD). The stability of N-Trp-mRBP appears to be the same as that of C-Trp-mRBP, another mutant obtained by relpacing Phe-187 with a Trp, and lower than that of WT-mRBP. The overall refolding rate of N-Trp-mRBP was much smaller than that of C-Trp-mRBP which ,in turn, is similar to that of WT-mRBP. For the case of WT-mRBP, the rate constant obtained by Tyr fluorescence was identical to the value obtained by CD. But with C-Trp- mRBP, the rate constant from CD was smaller than the value from the Trp- fluorescence and this difference in the rate constants was much greater with the N-Trp-mRBP. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 리폴딩 키네틱스 | - |
dc.subject | 평형 언폴딩 | - |
dc.subject | 도메인 폴딩 | - |
dc.subject | Mutant ribose binding protein | - |
dc.subject | Equilibrium unfolding | - |
dc.subject | Refolding kinetics | - |
dc.subject | Domain folding | - |
dc.subject | 리보스 결합 단백질 | - |
dc.title | Stability and folding of a mutant ribose binding protein of escherichia coli | - |
dc.title.alternative | 대장균의 돌연변이 리보스 결합 단백질의 안정성 및 폴딩에 관한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 112651/325007 | - |
dc.description.department | 한국과학기술원 : 생물과학과, | - |
dc.identifier.uid | 000953120 | - |
dc.contributor.localauthor | Kim, Hyoung-Man | - |
dc.contributor.localauthor | 김형만 | - |
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