Molecular cloning, sequencing and expression of a cDNA encoding guamerin = 거머린 cDNA의 분자클로닝, 염기서열 및 발현

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A cDNA encoding a guamerin has been cloned in E. coli and characterized. The guamerin is an elastase specific inhibitor with a low molecular weight of cysteine rich polypeptide isolated from Korean native leech, Hirudo nipponia. A cDNA encoding guamerin was identified by PCR amplification with degenerate primers based on its partial amino acid sequences of N-terminal and C-terminal. The deduced amino acid sequence revealed a different amino acid residue from that of guamerin. This cDNA had 71% local identity to cDNA of antistasin, factor Xa inhibitor derived from leech, Haementaria officinalis at the DNA level. The cDNA was expressed in E. coli and a 9-kDa protein was produced with inhibitory activity against neutrophil elastase as expected.
Advisors
Kang, Ke-WonKim, Sun-Chang강계원김선창
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
1997
Identifier
112646/325007 / 000953018
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 1997.2, [ vi, 47 p. ]

Keywords

Sequencing; Expression; cDNA; 거머린; 클로닝; 염기서열분석; 발현; cDNA; Guamerin; Cloning

URI
http://hdl.handle.net/10203/28509
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=112646&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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