Novel mechanism of catabolite repression that exists in the pentose operons of escherichia coli K-12 = 대장균 오탄당 오페론에 존재하는 새로운 대사산물 억제 기작

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 480
  • Download : 0
During the study on transcriptional regulation of Escherichia coli K-12 ribose (rbs) operon, it was observed that the operon is repressed by the presence of D-xylose. However, the other sugars examined except D-glucose had little effect on the expression of the rbs operon and the xylose(xyl) operon was not affected by the presence of D-ribose. Genetic analysis and β-galactosidase assay revealed that XylR, a regulator of the xylose operon, was responsible for the repression. {\it E.coli} cells also showed a diauxic growth, which is typical in the catabolite repression by D-glucose, in minimal medium containing D-xylose plus D-ribose. These results led us to the conclusion that there exists novel mechanism of catabolite repression in the rbs operon that is exerted by D-xylose through the action of XylR. Furthermore, it was shown that the xyl and rbs operon were repressed by the presence of L-arabinose whereas the arabinose operon was not affected by D-xylose or D-ribose, suggesting that there exists a mechanism determining the preference of pentose utilization at the level of transcription.
Advisors
Park, Chan-KyuresearcherChung, Jong-Kyoungresearcher박찬규researcher정종경researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
1997
Identifier
112645/325007 / 000953014
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 1997.2, [ iv, 53 p. ]

Keywords

Catabolite repression; Pentose; 대사산물억제; 오탄당

URI
http://hdl.handle.net/10203/28508
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=112645&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0