Isolation and characterization of mucin like protein from Korean leech skin = 거머리 피부 뮤신 단백질의 생화학적 연구

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Mucus from leech skin consists of mucin-like glycoproteins (designated as leech skin mucin) in association with several low molecular weight proteins and glycoproteins. A high molecular weight, heavily glycosylated protein fraction was isolated from leech skin by sequential chromatography on Bio-gel a-15m, Sepharose CL-2B. The isolated mucin comprsied about 80% carbohydrate and 20% protein. Threonine and Serine comprised 35% of the total amino acids while the oligosaccharide moiety contained glucosamine, galactosamine, fucose, galactose, glucose, mannose and sialic acid. Following alkaline borohydrate treatment, the carbohydrate chains were found to be linked O-glycosidically through threonine and serine. Leech mucin was analysed by CM52 cellose ion exchange chromatography and SDS PAGE. Its average molecular weight was estimated to about 480KDa.
Advisors
Kang, Ke-Won강계원
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
1996
Identifier
105879/325007 / 000943436
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 1996.2, [ 43 p. ]

Keywords

Leech; Mucin; Skin; Protein

URI
http://hdl.handle.net/10203/28503
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=105879&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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