Development of the pipeline for analysis of activated sludge metatranscriptomes활성 슬러지 메타전사체 분석을 위한 파이프라인 구축

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In the bioreactor of the wastewater treatment plant, pollutants such as nitrogen and sulfur are removed using microorganisms. Microorganisms in wastewater treatment plants are difficult to analyze due to a large number of inhibitors, particularly, in the case of a transcript, degradation starts immediately from the moment of departure from the environment, so an optimal method is required from the collecting step. This paper introduces a method for successfully collecting the genome and transcripts from microorganisms in wastewater and a method for analyzing them using various bioinformatics programs. By analyzing the results, the community of microorganisms in the wastewater was analyzed, and 13 metagenome-assembled genomes (MAGs) were identified to analyze their phylogenetic characteristics. Through these gene analyses, it was possible to know the biological reactions occurring in the wastewater, and to analyze the specific genes related to nitrogen cycle in more detail. And through transcriptome analysis, it is possible to know the activity of microorganisms at the time of sampling, not the general genetic characteristics, and compare the degree of expression for each gene. It can be applied to various studies using microorganisms in wastewater through the introduced method.
Advisors
Yoon, Sukhwanresearcher윤석환researcher
Description
한국과학기술원 :건설및환경공학과,
Publisher
한국과학기술원
Issue Date
2020
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 건설및환경공학과, 2020.8,[iii, 17 p. :]

Keywords

Environmental microorganism▼aNitrogen cycle▼aFunctional gene▼aMetagenome▼aMetatranscriptome; 환경미생물▼a질소순환▼a기능유전자▼a메타유전체▼a메타전사체

URI
http://hdl.handle.net/10203/285017
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=925178&flag=dissertation
Appears in Collection
CE-Theses_Master(석사논문)
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