DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kim, Sun-Chang | - |
dc.contributor.advisor | 김선창 | - |
dc.contributor.author | Kim, Hun-Sik | - |
dc.contributor.author | 김헌식 | - |
dc.date.accessioned | 2011-12-12T09:00:51Z | - |
dc.date.available | 2011-12-12T09:00:51Z | - |
dc.date.issued | 1996 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=105870&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28495 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물과학과, 1996.2, [ v, 47 p. ] | - |
dc.description.abstract | A cDNA containing coding information for buforin I (toad stomach antimicrobial peptide) was identified by PCR. The cloned cDNA codes for total 128 amino acids, contains buforin I, a single copy in the N-terminal region. Nucleotide sequence analysis of corresponding cDNA revealed that it shows significant homology with histone H2A, a replication-dependent histone of which expression of mRNAs is tightly coupled with DNA synthesis and involves both transcriptional and post-transcriptional mechanisms. Therefore, it is postulated that there exists a regulation mechanism to convert toad histone H2A.1 to buforin I that is also coupled with the cell cycle. A cDNA corresponding to TSAP (toad skin antimicrobial peptide) was also isolated using 3``-RACE and library screening. The cloned cDNA codes for total 102 amino acids, and TSAP is composed of 95 amino acids and 7 amino acids as putative signal sequence. Nucleotide sequence analysis of cloned cDNA shows homology with cysteine proteinase inhibitors, especially family 1 stefins (average 40%), implies its dual role in this organism. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Histone H2A | - |
dc.subject | cDNA Cloning | - |
dc.subject | Toad | - |
dc.subject | Cysteine Proteinase Inhibitor | - |
dc.subject | 시스테인 단백질 분해효소 저해제 | - |
dc.subject | 히스톤 H2A | - |
dc.subject | cDNA 클로닝 | - |
dc.subject | 두꺼비 | - |
dc.title | cDNA cloning and characterization of antimicrobial peptides from Bufo bufo gagarizans | - |
dc.title.alternative | 한국산 두꺼비 Bufo bufo gagarizans로부터 항균성 펩타이드의 cDNA Cloning및 Characterization | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 105870/325007 | - |
dc.description.department | 한국과학기술원 : 생물과학과, | - |
dc.identifier.uid | 000943146 | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.localauthor | 김선창 | - |
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