Laser-modulated Lissajous scanner for structured illumination microscopy = 구조광 현미경을 위한 레이저 변조 리사쥬 스캐너에 관한 연구

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High resolution microscopic imaging technique has been steadily demanded to observe detailed structures or dynamic mechanisms of biological samples. Structured illumination microscopy (SIM) has grabbed much attention due to its simplicity, compatibility with common fluorescent molecules, and fast image acquisition. Here we report Lissajous scanning SIM (LS-SIM) by using Lissajous scanning micromirror and laser intensity modulation. Biaxial scanning frequencies of Lissajous micromirror are substantially separated to avoid mechanical coupling and the fast axis inner mirror has a relatively low Q-factor to obtain a broad selection range of scanning frequency. A Lissajous trajectory of high fill-factor is achieved with a selected set of scanning frequencies at pseudo-resonance, where Lissajous scanned structured illumination (SI) is realized by modulating a laser beam at least common multiple (LCM) of bi-axial scanning frequencies. A compact Lissajous scanned SI module, containing fiber-optic collimator and Lissajous micromirror is fully packaged, coupled with fiber-based DPSS laser with acousto-optic-modulator (AOM) and mounted with a conventional fluorescent microscope. The LS-SIM clearly demonstrates high-resolution microscopic imaging after the image reconstruction of various imaging targets. This method will provide many potentials for diverse fluorescence microscopic applications.
Advisors
Jeong, Ki-Hunresearcher정기훈researcher
Description
한국과학기술원 :바이오및뇌공학과,
Publisher
한국과학기술원
Issue Date
2020
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2020.8,[v, 62 p. :]

Keywords

Structured illumination microscopy▼aMicromirror▼aLissajous scanner▼aHigh-resolution imaging; 구조광 현미경▼a마이크로 미러▼a리사쥬 스캐너▼a고해상도 이미징

URI
http://hdl.handle.net/10203/284929
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=925088&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
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