Loss of productivity of transfectoma producing chimeric antibody during a long-term culture = 트랜스펙토마의 장기 배양시 항체 생산력 감소에 관한 연구

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The stability of KR12H-1 transfectoma in regard to chimeric antibody production was examined during long-term, repeated batch culture without selection pressure using antibiotics. Both serum-supplemented and serum-free media were used. Regardless of the medium used, the specific antibody productivity ($q_{Ab}$) of transfectoma decreased by 60-88% during 70-day culture. This loss of antibody productivity was not due mainly to the appearance of a nonproducing population (NP) of transfectoma. The percentage of a producing population (P), which was monitored by the limiting dilution method, remained over 90% until the end of culture, indicating that the $q_{Ab}$ of P decreased during the culture. Flow cytometric data also showed the increase of cell population with low fluorescence intensity during culture, indicating that the intracellular antibody content of P decreased. The subclones of P obtained at the end of long-term culture were further characterized. Compared with the $q_{Ab}$ of P at the beginning of long-term culture, the $q_{Ab}$ of most P subclones was significantly low, confirming that the loss of antibody productivity was due mainly to the decreased $q_{Ab}$ of P during long-term culture. The decreased antibody gene copy number of P subclones was found to be partly responsible for the decreased $q_{Ab}$ of P during the long-term culture.
Advisors
Lee, Gyun-Minresearcher이균민researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
1996
Identifier
105845/325007 / 000943117
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 1996.2, [ vi, 42 p. ]

Keywords

Long-term culture; Stability; Chimeric antibody; RT-PCR; 장기배양; 안정성; 재조합 항체; Southern Blot

URI
http://hdl.handle.net/10203/28479
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=105845&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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