DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kang, Ke-Won | - |
dc.contributor.advisor | 강계원 | - |
dc.contributor.author | Jung, Hyo-Il | - |
dc.contributor.author | 정효일 | - |
dc.date.accessioned | 2011-12-12T09:00:34Z | - |
dc.date.available | 2011-12-12T09:00:34Z | - |
dc.date.issued | 1995 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=98710&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28476 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1995.2, [ v, 47 p. ] | - |
dc.description.abstract | Inhibitory peptides against protease were studied from a Korean native leech species, Hirudo nipponia. An elastase inhibitor was purified and characterized by using Sephadex G-75 gel filtration, anion exchange chromatography on DEAE-Sepharose and $C_{18}$ reversed phase HPLC column, followed by acetone precipitation and the complete amino acid sequence of the inhibitor was determined. The purified peptide has a molecular weight of 6,119 Da on MALDI mass spectrum. Inhibitory activity of the peptide was examined on the various kind of proteases. Though the inhibitory peptide has a small effect on chymotrypsin and subtilisin, no inhibitory effect on other proteases such as trypsin, pepsin, and papain was observed. The peptide is stable at the wide range of pH 1 to 11 and is heat stable up to $90^\circ C$. The sequence of the peptide was compared with other peptide inhibitors. The amino acid sequence of the peptide shows similarity to subtilisin - chymotrypsin inhibitors from barley and maize and Factor Xa inhibitor, antistasin, from Mexican leech. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | (A) leech-derived human leukocyte elastase inhibitor | - |
dc.title.alternative | 거머리에서 분리한 단백질 분해 효소 억제제에 관한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 98710/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000933472 | - |
dc.contributor.localauthor | Kang, Ke-Won | - |
dc.contributor.localauthor | 강계원 | - |
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