Specific cleavage and labeling of chromosomal DNA at a single situ using site-specific recombination = Site-specific recombination을 이용한 염색체 DNA의 특정위치에서의 절단 및 표지

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A novel method in cleavage of chromosomal DNA at a single site has been developed. Saccharomyces cerevisiae genome was precisely cleaved at a predetermined site using Cre/loxP recombination which mediates the sitespecific recombination between two loxP sites composed of 34bp each. At first, the loxP site was introduced into one of the 16 yeast chromosomes. The chromosomes were isolated from the resulting strain in a intact form and reacted with synthetic oligonucleotides containing loxP sites in the presence of Cre recombinase. By the recombination between two loxP sites on a chromosome and a oligonucleotide, the chromosome containing loxP sites was cleaved specifically. The cleavage was confirmed by Southern hybridization. When biotin-labeled oligonucleotides were used in reaction with plasmid DNA, the simultaneous cutting and labeling of DNA fragment could be accomplished.
Advisors
Kim, Sun-Changresearcher김선창researcher
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1995
Identifier
98709/325007 / 000933449
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1995.2, [ v, 46 p. ]

URI
http://hdl.handle.net/10203/28475
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=98709&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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